Difference between revisions of "Part:BBa K1940001"
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We designed this part to detect the abundance of microRNA-34c. Anti-microRNA-34c [https://parts.igem.org/Part:BBa_K1940000 (BBa_K1940000)] was insert after a constitutive promoter [https://parts.igem.org/Part:BBa_J23106 (BBa_J23106)] which express GFP. As anti- microRNA-34c is sensitive to the expression levels of miR-34c, we would have a direct impact on GFP’s expression. Determining GFP’s fluorescence intensity differences will justify the content amount of microRNA-34c.
 
We designed this part to detect the abundance of microRNA-34c. Anti-microRNA-34c [https://parts.igem.org/Part:BBa_K1940000 (BBa_K1940000)] was insert after a constitutive promoter [https://parts.igem.org/Part:BBa_J23106 (BBa_J23106)] which express GFP. As anti- microRNA-34c is sensitive to the expression levels of miR-34c, we would have a direct impact on GFP’s expression. Determining GFP’s fluorescence intensity differences will justify the content amount of microRNA-34c.
  
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[[File:Figure. 3：Promoter-antimicro-RBS.png|700px|]]
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[[File:Figure. 3：Promoter-antimicro-RBS.tif|700px|]]
  
 
'''Figure 1: Construct design.''' dCas9 was fused via a 3 amino acid linker to VP16. The resulting fusion construct was flanked by NLS sequences and tagged by a HA epitope. The CMV promoter and BGH terminator were chosen to control gene expression.   
 
'''Figure 1: Construct design.''' dCas9 was fused via a 3 amino acid linker to VP16. The resulting fusion construct was flanked by NLS sequences and tagged by a HA epitope. The CMV promoter and BGH terminator were chosen to control gene expression.   

Revision as of 06:10, 12 October 2016

microRNA-34c detector 1.0

We designed this part to detect the abundance of microRNA-34c. Anti-microRNA-34c (BBa_K1940000) was insert after a constitutive promoter (BBa_J23106) which express GFP. As anti- microRNA-34c is sensitive to the expression levels of miR-34c, we would have a direct impact on GFP’s expression. Determining GFP’s fluorescence intensity differences will justify the content amount of microRNA-34c.

File:Figure. 3：Promoter-antimicro-RBS.tif

Figure 1: Construct design. dCas9 was fused via a 3 amino acid linker to VP16. The resulting fusion construct was flanked by NLS sequences and tagged by a HA epitope. The CMV promoter and BGH terminator were chosen to control gene expression.

Sequence and Features

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Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 186
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 50
    Illegal BsaI.rc site found at 183
    Illegal BsaI.rc site found at 864

References