Difference between revisions of "Part:BBa K1890011"

 
Line 3: Line 3:
 
<partinfo>BBa_K1890011 short</partinfo>
 
<partinfo>BBa_K1890011 short</partinfo>
  
−
mVenus is a variant of yellow fluorescent proteins (YFP) which contains several mutations that make it more stable1 and provide an improved speed and efficiency of maturation. We expressed it under the control of the strong constitutive promoter J23100 and RBS B0034, both sequences were available in the iGEM registry.  
+
mVenus is a variant of yellow fluorescent protein (YFP) which contains several mutations that make it more stable and provide an improved speed and efficiency of maturation [1]. We expressed it under the control of the strong constitutive promoter <partinfo>BBa_J23100</partinfo> and RBS <partinfo>BBa_B0030</partinfo>.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 10:41, 8 October 2016


mVenus with strong consitutive promoter and RBS

mVenus is a variant of yellow fluorescent protein (YFP) which contains several mutations that make it more stable and provide an improved speed and efficiency of maturation [1]. We expressed it under the control of the strong constitutive promoter BBa_J23100 and RBS BBa_B0030.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]