Difference between revisions of "Part:BBa K592011"
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[[Image:cj blue swiss.png]]
 
[[Image:cj blue swiss.png]]
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QMEAN = -0.39
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<br>Cβ = -0.84
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<br>All Atom = 0.10
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<br>Solvation = -1.49
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<br>Torsion = -0.01
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<span class='h3bb'><strong>The best match during structure prediction.</strong></span>
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<table border="1">
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<tr>
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<th>Template</th>
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<th>Seq. Identity</th>
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<th>Oligo-state</th>
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<th>Found by</th>
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<th>Method</th>
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<th>Resolution</th>
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<th>Seq Similarity</th>
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<th>Range</th>
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<th>Coverage</th>
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<th>Description</th>
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</tr>
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<tr>
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<td>2ib5.1.A</td>
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<td>99.57</td>
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<td>homo-octamer</td>
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<td>BLAST</td>
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<td>X-Ray</td>
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<td>1.80Å</td>
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<td>0.63</td>
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<td>5 - 232</td>
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<td>0.99</td>
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<td>Chromo protein</td>
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</tr>
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</table>
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<strong>Ligands -</strong>None

Revision as of 13:14, 3 October 2016

cjBlue, green chromoprotein

This chromoprotein from the Cnidopus japonicus sea anemone, cjBlue, naturally exhibits dark green color when expressed. Compared to some other chromoproteins, such as amilCP (BBa_K592009), amilGFP (BBa_K592010), spisPink (BBa_K1033932), asPink (BBa_K1033933) and aeBlue (BBa_K864401), the color development is slower. The color is readily observed in both LB or on agar plates after 24-48 hours of incubation. The sequence is codon optimized for expression in E coli.

Usage and Biology

This part is useful as a reporter.

CjBlue plate small.jpg

iGEM11_Uppsala-Sweden: Expression of green chromoprotein. Escherichia coli constitutively expressing cjBlue (BBa_K592011).

UUChromo.jpg

iGEM12_Uppsala_University: The Uppsala chromoprotein collection and RFP. The image shows pellets of E coli expressing chromoproteins eforRed BBa_K592012, RFP BBa_E1010, cjBlue BBa_K592011, aeBlue BBa_K864401, amilGFP BBa_K592010 and amilCP BBa_K592009.

References

[http://www.jbc.org/content/281/49/37813.abstract] Chan MC, Karasawa S, Mizuno H, Bosanac I, Ho D, Privé GG, Miyawaki A, Ikura M. (2006) Structural characterization of a blue chromoprotein and its yellow mutant from the sea anemone Cnidopus japonicus. J. Biol. Chem. 281(49). 37813-9


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]



Structure and SWISS MODEL Homology Report

(The following information has been contributed by SVCE_Chennai 2016)

The following sections give a detailed information on this chromoprotein done by its in silico analysis.



3D Structure

Cjblueswiss.gif



Cartoon representation of cjBlue chromoprotein.

Cj blue swiss.png


QMEAN = -0.39
Cβ = -0.84
All Atom = 0.10
Solvation = -1.49
Torsion = -0.01


The best match during structure prediction.

Template Seq. Identity Oligo-state Found by Method Resolution Seq Similarity Range Coverage Description
2ib5.1.A 99.57 homo-octamer BLAST X-Ray 1.80Å 0.63 5 - 232 0.99 Chromo protein


Ligands -None