Difference between revisions of "Part:pSB3C5:Experience"

Revision as of 21:32, 3 May 2007

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of pSB3C5

User Reviews

UNIQ0afbabf81cfe7b75-partinfo-00000000-QINU

pSB3C5
Reshma Shetty

This plasmid seems to work. When transformed into TOP10 cells, pSB3C5-I52001 does not transform but when cut with NotI and self-ligated (to eliminate ccdB), lots of colonies are obtained. However, when miniprepped, pSB3C5-I52001 does not give as high of yields as expected for a high copy plasmid likely due to mutations in BBa_I52001 that render it nonfunctional as a high copy origin. I am working to address this issue. This plasmid should still work fine (it is just not as convenient to prep).

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 <I>Reshma Shetty</I>
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-This plasmid seems to work.  When transformed into TOP10 cells, pSB3C5-I52001 does not transform but when cut with NotI and self-ligated (to eliminate ccdB), lots of colonies are obtained.  However, when miniprepped, pSB3C5-I52001 does not give as high of yields as expected for a high copy plasmid likely due to mutations in BBa_I52001 that render it nonfunctional as a high copy origin.  I am working to address this issue.
+This plasmid seems to work.  When transformed into TOP10 cells, pSB3C5-I52001 does not transform but when cut with NotI and self-ligated (to eliminate ccdB), lots of colonies are obtained.  However, when miniprepped, pSB3C5-I52001 does not give as high of yields as expected for a high copy plasmid likely due to mutations in BBa_I52001 that render it nonfunctional as a high copy origin.  I am working to address this issue.  This plasmid should still work fine (it is just not as convenient to prep).
 |}
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