Difference between revisions of "Part:BBa K1602046"

Revision as of 07:28, 25 September 2015

RRK3

Composite part consisting of an araC-regulated pBAD-promoter (BBa_K808000), Key3 (BBa_J01086) and a terminator (BBa_B0015). It is half of a two-part riboregulator-system for E.coli for posttransciptional regulation of gene expression. Upon transcription the sequence of Key3(taRNA) forms a RNA-RNA-complex with corresponding cis-repressing sequences (crRNA). This leads to a helix shift and the release of the formerly masked ribosome binding site (RBS) enabling the expression of the regulated gene of interest (GOI). The corresponding crRNAs are RRL3H and RRL3G.

**Figure 1:** Interaction of taRNA and crRNA leads to gene expression.

Because of the araC-regulated pBAD-promoter production of the Lock3-sequence is induced by the addition of arabniose. In the presence of glucose araCpBAD shows a very low basal expression level. In combination with the corresponding crRNA-parts (RRL3H and RRL3G) this results in an induceable riboregulator-system which represses gene expression in the presence of glucose but is inducable through the addition of arabinose.

Functional Parameters

References

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 1144
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 979
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 1215
Illegal SapI site found at 961

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 <partinfo>BBa_K1602046 short</partinfo>
+Composite part consisting of an araC-regulated pBAD-promoter (<html><a href="/Part:BBa_K808000">BBa_K808000</a></html>), Key3 (<html><a href="/Part:BBa_J01086">BBa_J01086</a></html>) and a terminator (<html><a href="/Part:BBa_B0015">BBa_B0015</a></html>). It is half of a two-part riboregulator-system for <i>E.coli</i> for posttransciptional regulation of gene expression. Upon transcription the sequence of <html><a href="/Part:BBa_J01086">Key3</a></html>(taRNA) forms a RNA-RNA-complex with corresponding cis-repressing sequences (crRNA). This leads to a helix shift and the release of the formerly masked ribosome binding site (RBS) enabling the expression of the regulated gene of interest (GOI). The corresponding crRNAs are <html><a href="/Part:BBa_K1602044">RRL3H</a></html> and <html><a href="/Part:BBa_K1602045">RRL3G</a></html>.
+<html>
+<center>
+<figure >
+	<img width=30%; src="https://static.igem.org/mediawiki/parts/3/3d/Schema_GOI.png">
+	<figcaption><b>Figure 1:</b> Interaction of taRNA and crRNA leads to gene expression.</figcaption>
+</figure>
+</center>
+</html>
+Because of the <html><a href="/Part:BBa_K808000">araC-regulated pBAD-promoter</a></html> production of the Lock3-sequence is induced by the addition of arabniose. In the presence of glucose araCpBAD shows a very low basal expression level. In combination with the corresponding crRNA-parts (<html><a href="/Part:BBa_K1602044">RRL3H</a></html> and <html><a href="/Part:BBa_K1602045">RRL3G</a></html>) this results in an induceable riboregulator-system which represses gene expression in the presence of glucose but is inducable through the addition of arabinose.