Difference between revisions of "Part:BBa K1602043"

Revision as of 05:47, 25 September 2015

araCpBad-hokD

Composite part consisting of an araC-regulated pBAD-promoter (BBa_K808000) cloned upstream of a ribosome binding site (BBa_B0034) and hokD (BBa_K1497008). Because of the araC-regulated pBAD-promoter production of the hokD is induced by the addition of arabniose which leads to cell death of E.coli. In the presence of glucose araCpBAD shows a very low basal expression level and therefore prevents the fatal expression of the toxin.

Functional Parameters

References

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 1144
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 979
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI site found at 961

@@ Line 2: / Line 2: @@
 <partinfo>BBa_K1602043 short</partinfo>
-Composite part consisting of an araC-regulated pBAD-promoter (<html><a href="/Part:BBa_K808000">BBa_K808000</a></html>) cloned upstream of a ribosome binding site (<html><a href="/Part:BBa_B0034">BBa_B0034</a></html>) and hokD (<html><a href="/Part:BBa_K1497008">BBa_K1497008</a></html>).
+Composite part consisting of an araC-regulated pBAD-promoter (<html><a href="/Part:BBa_K808000">BBa_K808000</a></html>) cloned upstream of a ribosome binding site (<html><a href="/Part:BBa_B0034">BBa_B0034</a></html>) and hokD (<html><a href="/Part:BBa_K1497008">BBa_K1497008</a></html>). Because of the <html><a href="/Part:BBa_K808000">araC-regulated pBAD-promoter</a></html> production of the hokD is induced by the addition of arabniose which leads to cell death of <i>E.coli</i>. In the presence of glucose araCpBAD shows a very low basal expression level and therefore prevents the fatal expression of the toxin.
 ===Functional Parameters===