Difference between revisions of "Part:BBa K1616005"
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<partinfo>BBa_K1616005 short</partinfo>
 
<partinfo>BBa_K1616005 short</partinfo>
  
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<h3> <font style="color:#b22222">Holin - Endolysin</font> </h3>
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<br>
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H/E
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Holin and endolysin are <b>toxins</b>; after a period of late-gene expression, holin, an inner permeabilizing protein creates holes in the membrane in order to permeabilize it and then endolysin, a muralytic enzyme traverses the holes creating by holin and degraded the wall cell. This complex induce cell lysis of bacteria. (Young & Bläsi, 1995)  
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Holin and endolysin are toxins; after a period of late-gene expression, holin, an inner permeabilizing protein creates holes in the membrane in order to permeabilize it and then endolysin, a muralytic enzyme traverses the holes creating by holin and degraded the wall cell. This complex induce cell lysis of bacteria. (Young & Bläsi, 1995)  
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===Design Notes===
 
===Design Notes===
 
All BioBrick parts used for assembling the composite part are compatible with the RFC10 Biobrick standard.
 
All BioBrick parts used for assembling the composite part are compatible with the RFC10 Biobrick standard.
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===Source===
 
===Source===
  
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BBa_K1616005 is a composite part with BBa_K1616006 (endolysin reversed), BBa_K1616007 (reversed), BBa_K1616011 (RBS reversed) and BBa_B0025 (Double terminator T7 reversed).  
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BBa_K1616005 is a composite part with <bbpart>BBa_K1616006</bbpart> (endolysin reversed), <bbpart>BBa_K1616007</bbpart> (reversed), <bbpart>BBa_K1616011</bbpart> (RBS reversed) and <bbpart>BBa_B0025</bbpart> (double terminator T7 reversed).  
  
 
===References===
 
===References===
  
 
Young, R., & Bläsi, U. (1995). Holins: Form and function in bacteriophage lysis. In FEMS Microbiology Reviews (Vol. 17, pp. 191–205). http://doi.org/10.1016/01686445(94)00079-4
 
Young, R., & Bläsi, U. (1995). Holins: Form and function in bacteriophage lysis. In FEMS Microbiology Reviews (Vol. 17, pp. 191–205). http://doi.org/10.1016/01686445(94)00079-4

Revision as of 17:21, 21 September 2015

Holin/endolysin reversed - RBS and double T7 terminator

Holin - Endolysin


Holin and endolysin are toxins; after a period of late-gene expression, holin, an inner permeabilizing protein creates holes in the membrane in order to permeabilize it and then endolysin, a muralytic enzyme traverses the holes creating by holin and degraded the wall cell. This complex induce cell lysis of bacteria. (Young & Bläsi, 1995)



Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 306
    Illegal AgeI site found at 376
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

All BioBrick parts used for assembling the composite part are compatible with the RFC10 Biobrick standard.


Source

BBa_K1616005 is a composite part with BBa_K1616006 (endolysin reversed), BBa_K1616007 (reversed), BBa_K1616011 (RBS reversed) and BBa_B0025 (double terminator T7 reversed).

References

Young, R., & Bläsi, U. (1995). Holins: Form and function in bacteriophage lysis. In FEMS Microbiology Reviews (Vol. 17, pp. 191–205). http://doi.org/10.1016/01686445(94)00079-4