Difference between revisions of "Part:BBa K1583015"

 
 
(6 intermediate revisions by the same user not shown)
Line 1: Line 1:
−
 
 
__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K1583015 short</partinfo>
 
<partinfo>BBa_K1583015 short</partinfo>
 +
<html>
 +
<p>A linker with the protein sequence GSGGSG was placed in between the CsgA (<a href="https://parts.igem.org/Part:BBa_K1583000"target="_blank">BBa_K1583000</a>) and the hydroxyapatite-affinity tag (<a href="https://parts.igem.org/Part:BBa_K1583102"target="_blank">BBa_K1583102</a>). This design was based on the paper "Programmable biofilm-based materials from engineered curli nanofibres" </p>
  
−
A linker with the protein sequence GSGGSG was placed in between the CsgA and the affinity tag for increased performance reasons. The design was based on the paper "Programmable biofilm-based materials from
+
</html>
−
engineered curli nanofibres"[1]
+
−
 
+
−
1. Nguyen, P. Q., Botyanszki, Z., Tay, P. K. R., & Joshi, N. S. (2014). Programmable biofilm-based materials from engineered curli nanofibres. Nature communications, 5.
+
−
 
+
−
<!-- Add more about the biology of this part here
+
−
===Usage and Biology===
+
−
 
+
 
<!-- -->
 
<!-- -->
−
<span class='h3bb'>Sequence and Features</span>
+
<span class='h3bb'><h3>Sequence and Features</h3></span>
 
<partinfo>BBa_K1583015 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K1583015 SequenceAndFeatures</partinfo>
  
Line 20: Line 14:
 
<partinfo>BBa_K1583015 parameters</partinfo>
 
<partinfo>BBa_K1583015 parameters</partinfo>
 
<!-- -->
 
<!-- -->
 +
<h3>References</h3>
 +
<p>Nguyen, P. Q., Botyanszki, Z., Tay, P. K. R., & Joshi, N. S. (2014). Programmable biofilm-based materials from engineered curli nanofibres. Nature communications, 5.</p>

Latest revision as of 01:08, 19 September 2015

Linker in fusion protein

A linker with the protein sequence GSGGSG was placed in between the CsgA (BBa_K1583000) and the hydroxyapatite-affinity tag (BBa_K1583102). This design was based on the paper "Programmable biofilm-based materials from engineered curli nanofibres"

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


References

Nguyen, P. Q., Botyanszki, Z., Tay, P. K. R., & Joshi, N. S. (2014). Programmable biofilm-based materials from engineered curli nanofibres. Nature communications, 5.