Difference between revisions of "Part:BBa K1638007"
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<partinfo>BBa_K1638007 short</partinfo> | <partinfo>BBa_K1638007 short</partinfo> | ||
− | This part codes for a GFP reporter with a flexible 10 aa linker at N-terminus. Intended for use in protein-GFP fusions, where this part can be suffixed to various proteins. The protein-GFP fusion can be used as a reporter of gene expression. | + | This part codes for a green fluorescent protein (GFP) reporter with a flexible 10 aa linker at N-terminus. Intended for use in protein-GFP fusions, where this part can be suffixed to various proteins. The protein-GFP fusion can be used as a reporter of gene expression and used in localization assays. A TEV-protease recognition site enables separation of the two proteins. This can be useful in different occasions, e.g. protein purification. |
− | This part also contains two transcriptional terminators. | + | This part also contains two transcriptional terminators. |
====Fluorescence wavelengths==== | ====Fluorescence wavelengths==== |
Revision as of 10:56, 18 September 2015
GFP reporter with flexible linker
This part codes for a green fluorescent protein (GFP) reporter with a flexible 10 aa linker at N-terminus. Intended for use in protein-GFP fusions, where this part can be suffixed to various proteins. The protein-GFP fusion can be used as a reporter of gene expression and used in localization assays. A TEV-protease recognition site enables separation of the two proteins. This can be useful in different occasions, e.g. protein purification. This part also contains two transcriptional terminators.
Fluorescence wavelengths
- Excitation max - 501nm
- Emission max - 511nm
For further description of the GFP read here.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 674