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This part is used in the 2014 LMU-Munich iGEM project [http://2014.igem.org/Team:LMU-Munich BaKillus].
  
 
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Latest revision as of 20:51, 17 October 2014

PxylA, a xylose inducible promoter for Bacillus subtilis

PxylA is the native promoter of the Bacillus subtilis xylose degradation locus xyl. The promoter is regulated by the repressor XylR which is not encoded by this BioBrick. The regulation of PxylA is only ensured, if you are working with B. subtilis as a chassis.

The BioBrick was evaluated with the integrative vector pBS3Clux, so the promoter activity was measured via luminescence output in B. subtilis.

The data (fig.1) clearly shows that PxylA can be gradually induced about 100-fold over background.

LMU14 PxylA-activity1.png

Fig. 1: Promoter activity of PxylA depending on the xylose concentration (0% to 0.2%). Panels A and D show cell density, B and E luminescence intensity (normalized by cell density). Panels C and F show dose-response curves one hour post-induction. Data derive from three independent experiments.



This part is used in the 2014 LMU-Munich iGEM project [http://2014.igem.org/Team:LMU-Munich BaKillus].

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 247
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]