Difference between revisions of "Part:BBa K1351039"
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P<sub><i>xylA</i></sub> is the native promoter of the ''Bacillus subtilis'' xylose degradation locus ''xyl''. The promoter is regulated by the repressor XylR which is not encoded by this BioBrick. The regulation of P<sub>''xylA''</sub> is only ensured, if you are working with ''B. subtilis'' as a chassis.
 
P<sub><i>xylA</i></sub> is the native promoter of the ''Bacillus subtilis'' xylose degradation locus ''xyl''. The promoter is regulated by the repressor XylR which is not encoded by this BioBrick. The regulation of P<sub>''xylA''</sub> is only ensured, if you are working with ''B. subtilis'' as a chassis.
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The BioBrick was evaluated with the integrative vector [https://parts.igem.org/wiki/index.php?title=Part:BBa_K823025 pBS3C''lux''], so the promoter activity was measured via luminescence output in ''B. subtilis''.
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The data (fig.1) clearly shows that P<sub><i>xylA</i></sub> can be gradually induced about 100-fold over background.
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<font color="#000000"; size="2"><p align="justify">'''Fig. 1: Promoter activity of P<sub>''xylA''</sub> depending on the xylose concentration (0% to 0.2%). Panels A and D show cell density, B and E luminescence intensity (normalized by cell density). Panels C and F show dose-response curves one hour post-induction. Data derive from three independent experiments.</p></font>
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Revision as of 17:47, 17 October 2014

PxylA, a xylose inducible promoter for Bacillus subtilis

PxylA is the native promoter of the Bacillus subtilis xylose degradation locus xyl. The promoter is regulated by the repressor XylR which is not encoded by this BioBrick. The regulation of PxylA is only ensured, if you are working with B. subtilis as a chassis.

The BioBrick was evaluated with the integrative vector pBS3Clux, so the promoter activity was measured via luminescence output in B. subtilis.

The data (fig.1) clearly shows that PxylA can be gradually induced about 100-fold over background.

400px

Fig. 1: Promoter activity of PxylA depending on the xylose concentration (0% to 0.2%). Panels A and D show cell density, B and E luminescence intensity (normalized by cell density). Panels C and F show dose-response curves one hour post-induction. Data derive from three independent experiments.




Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 247
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]