Difference between revisions of "Part:BBa K896008:Experience"
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| − | lac promoter ([https://parts.igem.org/Part:BBa_R0010 BBa_R0010]) in the upper of the was removed from BBa_K896008 ([https://parts.igem.org/Part:BBa_K1342005 | + | lac promoter ([https://parts.igem.org/Part:BBa_R0010 BBa_R0010]) in the upper of the zinTp was removed from BBa_K896008 resulting to new parts ([https://parts.igem.org/wiki/index.php?title=Part:BBa_K1342005 BBa_K1342005]). |
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Latest revision as of 15:00, 17 October 2014
- Before 60 min, the fluorescence level of zinTp+GFP steadily increased, while BBa_I13522 (positive control) remained stable around 35000 au as time gone by. At 60 min, zinTp reached its maximum transcription rate so the fluorescence level stop increasing around 55000 au.
- This fig shows the fluorescence level of different cadmium concentration. We can see that BBa_I13522 remained stable from different cadmium concentration, while the fluorescence level of zinTp+GFP increased as the cadmium concentration increased. We could also tell that the transcription rate of zinTp is fairly low when there is no cadmium ion.
Applications of BBa_K896008
It can be used to detect cadmium ion when ligating with GFP.
User Reviews
UNIQd401ad6db99818aa-partinfo-00000000-QINU
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No review score entered. iGEM Nagahama_2014 |
lac promoter (BBa_R0010) in the upper of the zinTp was removed from BBa_K896008 resulting to new parts (BBa_K1342005). |
UNIQd401ad6db99818aa-partinfo-00000002-QINU