Difference between revisions of "Part:BBa K592010"
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This chromoprotein from the coral ''Acropora millepora'', amilGFP, naturally exhibits strong yellow color when expressed. The color is readily visible to naked eye both in LB-culture and on agar plates. Color development can be seen in less than 24 hours of incubation. | This chromoprotein from the coral ''Acropora millepora'', amilGFP, naturally exhibits strong yellow color when expressed. The color is readily visible to naked eye both in LB-culture and on agar plates. Color development can be seen in less than 24 hours of incubation. | ||
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'''Important: This part is not available in the registry yet, however, the same part is available from the registry with the standard RBS B0034: <partinfo>BBa_K1033931</partinfo>.''' | '''Important: This part is not available in the registry yet, however, the same part is available from the registry with the standard RBS B0034: <partinfo>BBa_K1033931</partinfo>.''' | ||
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===Usage and Biology=== | ===Usage and Biology=== | ||
Revision as of 12:50, 12 June 2014
amilGFP, yellow chromoprotein
This chromoprotein from the coral Acropora millepora, amilGFP, naturally exhibits strong yellow color when expressed. The color is readily visible to naked eye both in LB-culture and on agar plates. Color development can be seen in less than 24 hours of incubation.
Important: This part is not available in the registry yet, however, the same part is available from the registry with the standard RBS B0034: BBa_K1033931.
Usage and Biology
This part is useful as a reporter.
iGEM11_Uppsala-Sweden: Expression of chromoproteins. The images above show E coli constitutively expressing amilGFP BBa_K592010 (yellow), amilCP BBa_K592009 (blue), and RFP BBa_E1010 (red).
iGEM12_Uppsala_University: The Uppsala chromoprotein collection and RFP. The image shows pellets of E coli expressing chromoproteins eforRed BBa_K592012, RFP BBa_E1010, cjBlue BBa_K592011, aeBlue BBa_K864401, amilGFP BBa_K592010 and amilCP BBa_K592009.
References
[http://www.ncbi.nlm.nih.gov/pubmed/18648549] Alieva, N. O., et al. 2008. Diversity and evolution of coral fluorescent proteins. PLoS One 3:e2680.
GenBank: AY646067.1
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]