Difference between revisions of "Part:BBa K1114016"

 
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__NOTOC__
 
__NOTOC__
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<partinfo>BBa_K1114016 short</partinfo>
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===Owl Trial===
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<html>
 +
    <head>
 +
        <title class="name"></title>
 +
        <link rel="shortcut icon" href="images/logo-Owl-Color_cropped.ico">
 +
        <link href="css/bootstrap.min.css" rel="stylesheet" media="screen">
 +
        <link href="css/bootstrap-responsive.min.css" rel="stylesheet" media="screen">
  
 +
        <meta http-equiv="Content-Type" content="text/html; charset=UTF-8">
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    </head>
 +
    <body class="container">
 +
        <!--summary-->
 +
        <div class="well" id ="basicInformation">
 +
           
 +
            <h1 class="name"></h1>
 +
            <h4>Part Description</h4>  This is a promoter from the Anderson Promoter library in MoClo format. This is a Level 0 MoClo part with flanking sites A on the 5' side and site B on the 3' side of the part. The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA.
  
 +
Summary of modifications from original part:
  
 +
Backbone is a modified version of pSB1C3 with added SpeI site in front of gene, BsaI sites flanking, and 4bp fusion sites. See BBa_K783055 .             
 +
            <h4>Summary</h4>
 +
           
 +
            <div class="row-fluid">
 +
                <div class="span6">
 +
                    <p id="summary"></p>
 +
                </div>
 +
                <div class="span6" id="deviceImage">
 +
                    <img src="" class="img-polaroid"/>
 +
                </div>
 +
            </div>
 +
           
 +
            <h4>Contact Information</h4>
 +
            <div class="row-fluid">               
 +
                <div class="span4">                                       
 +
                    <table>
 +
                        <tbody>                       
 +
                            <tr><th align="left">Author(s)</th><td id="authors"></td></tr>
 +
                            <tr><th align="left">Date</th><td id="date"></td></tr>
 +
                            <tr><th align="left">Team</th><td id="team"></td></tr>                     
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                        </tbody>   
 +
                    </table>
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                </div>
 +
               
 +
                <div class="span6">
 +
                    <table>
 +
                        <tbody>                       
 +
                            <tr><th align="left">Data Collection</th><td id="dataCollection"></td></tr>
 +
                            <tr><th align="left">Affiliation</th><td id="affiliation"></td></tr>
 +
                            <tr><th align="left">Contact</th><td id="contact"></td></tr>                     
 +
                        </tbody>   
 +
                    </table>
 +
                </div>
 +
            </div>
 +
        </div>
 +
        <hr/>
 +
       
 +
        <div class="well" id ="designDetails">
 +
           
 +
            <h4>Design Details</h4>
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            <div class="row-fluid">             
 +
                <div class="span4">
 +
                    <table>
 +
                        <tbody>
 +
                            <tr><th align="left">Type</th><td id="type"></td></tr>
 +
                            <tr><th align="left">Design Components</th><td id="designComponents"></td></tr>
 +
                        </tbody>
 +
                    </table>
 +
                </div>   
 +
 +
                <div class="span6">
 +
                    <table>
 +
                        <tbody>
 +
                            <tr><th align="left">Vector</th><td id="vector"></td></tr>
 +
                            <tr><th align="left">Additional Comments</th><td id="designComments"></td></tr>
 +
                        </tbody>   
 +
                    </table>
 +
                </div> 
 +
            </div> 
 +
        </div>
 +
        <hr/>
 +
       
 +
        <div class="well" id ="assemblyInformation">
 +
            <h4>Assembly Information</h4>
 +
            <div class="row-fluid">
 +
                <div class="span4">
 +
                    <table>
 +
                        <tbody>
 +
                            <tr><th align="left">Assembly Method(s)</th><td id="assemblyMethod"></td></tr>
 +
                            <tr><th align="left">Assembly RFC</th><td id="assemblyRFC"></td></tr>
 +
                            <tr><th align="left">Scars</th><td id="assemblyScars"></td></tr>
 +
                            <tr><th align="left">Assembly Components</th><td id="assemblyComponents"></td></tr>
 +
                        </tbody>
 +
                    </table>
 +
                </div>   
 +
 +
                <div class="span6">
 +
                    <table>
 +
                        <tbody>
 +
                            <tr><th align="left">Assembly Graph</th><td id="assemblyImage"></td></tr>
 +
                            <tr><th align="left">Chassis</th><td id="chassis"></td></tr>
 +
                            <tr><th align="left">Strain</th><td id="strain"></td></tr>                         
 +
                            <tr><th align="left">Additional Comments </th><td id="assemblyComments"></td></tr>                       
 +
                        </tbody>   
 +
                    </table>
 +
                </div> 
 +
            </div>
 +
            <hr/>
 +
        </div>
 +
 +
 +
        <div class="well">
 +
            <h4>Restriction Map</h4>
 +
            <div class="row-fluid">
 +
                <div id="restrictionMap" class="span6">
 +
                </div>
 +
            </div>
 +
        </div>
 +
        <hr/>
 +
 +
        <div class="well">
 +
            <h4>Flow Cytometry Experiment</h4>
 +
            <div class="row-fluid">
 +
                <div id="flowCytometry" class="span6">
 +
                </div>
 +
            </div>
 +
        </div>   
 +
        <hr/>   
 +
 +
 +
 +
 +
 +
 +
 +
 +
 +
<partinfo>BBa_K1114016 short</partinfo>
  
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===Design Notes===
 
 
<html>
 
<html>
 
This is the <a href="http://2013.igem.org/Team:BostonU/MoCloChara">MoClo</a> formatted part of BBa_J23113 with AB fusion sites. See the <a href="https://parts.igem.org/Part:BBa_J23113">BBa_J23113</a> page for full information on the part.  
 
This is the <a href="http://2013.igem.org/Team:BostonU/MoCloChara">MoClo</a> formatted part of BBa_J23113 with AB fusion sites. See the <a href="https://parts.igem.org/Part:BBa_J23113">BBa_J23113</a> page for full information on the part.  
 +
<br><br>
  
  
 
This is a Level 0 MoClo part with flanking sites A on the 5' side and site B on the 3' side of the part.
 
This is a Level 0 MoClo part with flanking sites A on the 5' side and site B on the 3' side of the part.
 
The fusion site letters refer to 4bp fusion sites: A        = GGAG;        B        = TACT; C        = AATG; D        = AGGT;        E        = GCTT; F        = CGCT; G        = TGCC; H        = ACTA.
 
The fusion site letters refer to 4bp fusion sites: A        = GGAG;        B        = TACT; C        = AATG; D        = AGGT;        E        = GCTT; F        = CGCT; G        = TGCC; H        = ACTA.
 +
<br><br>
  
 
Summary of modifications from original part:
 
Summary of modifications from original part:
 +
<br><br>
  
 
Backbone is a modified version of pSB1C3 with added SpeI site in front of gene, BsaI sites flanking, and 4bp fusion sites.  See <a href="https://parts.igem.org/Part:BBa_K783055">BBa_K783055 </a>.
 
Backbone is a modified version of pSB1C3 with added SpeI site in front of gene, BsaI sites flanking, and 4bp fusion sites.  See <a href="https://parts.igem.org/Part:BBa_K783055">BBa_K783055 </a>.
 +
  
 
</html>
 
</html>
  
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===Source===
 
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iGEM Distribution Kit
 
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===References===
 
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<!-- Add more about the biology of this part here
 
 
===Usage and Biology===
 
===Usage and Biology===
  
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<!-- -->
 
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<span class='h3bb'>Sequence and Features</span>
 
 
<partinfo>BBa_K1114016 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K1114016 SequenceAndFeatures</partinfo>
 +
 +
===Source===
 +
iGEM Distribution Kit
  
  
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<!-- Uncomment this to enable Functional Parameter display
 
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===Functional Parameters===
 
 
<partinfo>BBa_K1114016 parameters</partinfo>
 
<partinfo>BBa_K1114016 parameters</partinfo>
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<!-- -->
 

Latest revision as of 23:36, 18 January 2014

Owl Trial

Part Description

This is a promoter from the Anderson Promoter library in MoClo format. This is a Level 0 MoClo part with flanking sites A on the 5' side and site B on the 3' side of the part. The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA. Summary of modifications from original part: Backbone is a modified version of pSB1C3 with added SpeI site in front of gene, BsaI sites flanking, and 4bp fusion sites. See BBa_K783055 .

Summary

Contact Information

Author(s)
Date
Team
Data Collection
Affiliation
Contact

Design Details

Type
Design Components
Vector
Additional Comments

Assembly Information

Assembly Method(s)
Assembly RFC
Scars
Assembly Components
Assembly Graph
Chassis
Strain
Additional Comments

Restriction Map

Flow Cytometry Experiment

BBa_K1114016 short This is the MoClo formatted part of BBa_J23113 with AB fusion sites. See the BBa_J23113 page for full information on the part.

This is a Level 0 MoClo part with flanking sites A on the 5' side and site B on the 3' side of the part. The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA.

Summary of modifications from original part:

Backbone is a modified version of pSB1C3 with added SpeI site in front of gene, BsaI sites flanking, and 4bp fusion sites. See BBa_K783055 .

Usage and Biology


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 11
    Illegal NheI site found at 34
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Source

iGEM Distribution Kit