Difference between revisions of "Part:BBa K1033931"

 
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<partinfo>BBa_K1033999 short</partinfo>
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<partinfo>BBa_K1033931 short</partinfo>
  
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This chromoprotein from the coral Anemonia sulcata, asCP, naturally exhibits color when expressed.
 
  
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This chromoprotein from the coral ''Acropora millepora'', amilGFP, naturally exhibits strong yellow color when expressed. The color is readily visible to naked eye both in LB-culture and on agar plates. Color development can be seen in less than 24 hours of incubation.
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===Usage and Biology===
 
===Usage and Biology===
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This part is useful as a reporter.
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[[Image:AmilCP_amilGFP_RFP.jpg|300px]]
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[[Image:On cultures BYR small.jpg|300px]]
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[[Image:Pellets BYR.jpg|300px]]
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'''iGEM11_Uppsala-Sweden:''' Expression of chromoproteins. The images above show ''E coli'' constitutively expressing amilGFP <partinfo>BBa_K592010</partinfo> (yellow), amilCP <partinfo>BBa_K592009</partinfo> (blue), and RFP <partinfo>BBa_E1010</partinfo> (red).
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[[Image:UUChromo.jpg|600px]]
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'''iGEM12_Uppsala_University:''' The Uppsala chromoprotein collection and RFP. The image shows pellets of ''E coli'' expressing chromoproteins eforRed <partinfo>BBa_K592012</partinfo>, RFP <partinfo>BBa_E1010</partinfo>, cjBlue <partinfo>BBa_K592011</partinfo>, aeBlue <partinfo>BBa_K864401</partinfo>, amilGFP <partinfo>BBa_K592010</partinfo> and amilCP <partinfo>BBa_K592009</partinfo>.
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===Source===
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''Acropora millepora''
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===References===
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[http://www.ncbi.nlm.nih.gov/pubmed/18648549] Alieva, N. O., et al. 2008. ''Diversity and evolution of coral fluorescent proteins.'' PLoS One 3:e2680.
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GenBank: AY646067.1
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K1033999 SequenceAndFeatures</partinfo>
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<partinfo>BBa_K1033931 SequenceAndFeatures</partinfo>
  
  
 
<!-- Uncomment this to enable Functional Parameter display  
 
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===Functional Parameters===
 
===Functional Parameters===
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<partinfo>BBa_K1033999 parameters</partinfo>
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<partinfo>BBa_K1033931 parameters</partinfo>
 
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Latest revision as of 13:03, 8 November 2013

amilGFP, yellow chromoprotein (incl RBS)


This chromoprotein from the coral Acropora millepora, amilGFP, naturally exhibits strong yellow color when expressed. The color is readily visible to naked eye both in LB-culture and on agar plates. Color development can be seen in less than 24 hours of incubation.

Usage and Biology

This part is useful as a reporter.

AmilCP amilGFP RFP.jpg On cultures BYR small.jpg Pellets BYR.jpg

iGEM11_Uppsala-Sweden: Expression of chromoproteins. The images above show E coli constitutively expressing amilGFP BBa_K592010 (yellow), amilCP BBa_K592009 (blue), and RFP BBa_E1010 (red).

UUChromo.jpg

iGEM12_Uppsala_University: The Uppsala chromoprotein collection and RFP. The image shows pellets of E coli expressing chromoproteins eforRed BBa_K592012, RFP BBa_E1010, cjBlue BBa_K592011, aeBlue BBa_K864401, amilGFP BBa_K592010 and amilCP BBa_K592009.

Source

Acropora millepora

References

[http://www.ncbi.nlm.nih.gov/pubmed/18648549] Alieva, N. O., et al. 2008. Diversity and evolution of coral fluorescent proteins. PLoS One 3:e2680.

GenBank: AY646067.1


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]