Difference between revisions of "Part:BBa K1139026:Design"
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===Source=== | ===Source=== | ||
− | + | <i>BAD</i> promoter from (<partinfo>BBa_I13453</partinfo>), M13 genes and origin from <partinfo>BBa_K1139018</partinfo>, <i>GFP</i> from <partinfo>BBa_E0040</partinfo>, rbs from <partinfo>BBa_B0030</partinfo>, Double terminator from <partinfo>BBa_B0015</partinfo> are combined. | |
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===References=== | ===References=== |
Revision as of 16:37, 28 October 2013
Pbad-M13-Plac-GFP on pSB3
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 125
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 238
Illegal BamHI site found at 65 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 6164
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 7425
Design Notes
sequence confirmed
Source
BAD promoter from (BBa_I13453), M13 genes and origin from BBa_K1139018, GFP from BBa_E0040, rbs from BBa_B0030, Double terminator from BBa_B0015 are combined.