Difference between revisions of "Part:BBa K1073028:Experience"

Latest revision as of 09:29, 17 October 2013

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K1073028

By adding autoinducer N-butyryl-HSL to the media the expression of beta lactamase can be induced. The iGEM Team Braunschweig 2013 used this device in combination with BBa_K1073032 for the construction of BBa_K1073035.

User Reviews

UNIQ747a49cdb9f8c575-partinfo-00000002-QINU

BBa_K1073028 iGEM Team Braunschweig 2013

This device was used for the construction of BBa_K1073035. With this construct in a high copy plasmid (pSB1C3) we were able to induce expression of beta lactamase in E. coli Top10F' when N-butyryl-HSL was added to the media and thus maintain cell growth in complex medium containing ampicillin. For detailed experience see BBa_K1073035

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 This experience page is provided so that any user may enter their experience using this part.<BR>Please enter
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 ===Applications of BBa_K1073028===
-The iGEM Team Braunschweig 2013 used this device in combination with <bbpart>BBa_K1073032</bbpart> for the construction of <bbpart>BBa_K1073035</bbpart>. By adding autoinducer molecules N-buturyl-HSL to the media the expression of beta lactamase can be induced.
+By adding autoinducer N-butyryl-HSL to the media the expression of beta lactamase can be induced.
+The iGEM Team Braunschweig 2013 used this device in combination with <bbpart>BBa_K1073032</bbpart> for the construction of <bbpart>BBa_K1073035</bbpart>.
 ===User Reviews===
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 <I>iGEM Team Braunschweig 2013</I>
 |width='60%' valign='top'|
-This device was used for the construction of <bbpart>BBa_K1073035</bbpart>. With this construct in a high copy plasmid (pSB1C3) we were able to induce expression of beta lactamase in ''E. coli Top10'' when N-buturyl-HSL was added to the media and thus maintain cells growth in complex medium containing ampicillin. For detailed experience see <bbpart>BBa_K1073035</bbpart>
+This device was used for the construction of <bbpart>BBa_K1073035</bbpart>. With this construct in a high copy plasmid (pSB1C3) we were able to induce expression of beta lactamase in ''E. coli Top10F''' when N-butyryl-HSL was added to the media and thus maintain cell growth in complex medium containing ampicillin. For detailed experience see <bbpart>BBa_K1073035</bbpart>
 |};
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 <!-- DON'T DELETE --><partinfo>BBa_K1073028 EndReviews</partinfo>