Difference between revisions of "Part:BBa K1073014"

Latest revision as of 08:59, 17 October 2013

Constitutively expressed transcription regulator RhlR + LVA

This device consists of a strong constitutive promoter (BBa_J23100) combined with RBS (BBa_B0032) and the coding region of the transcription regulator RhlR (BBa_C0071). This results in a constitutively high transcription level of RhlR.

Usage and Biology

In combination with N-butyryl homoserine lactone (N-butyryl-HSL) synthesized by RhlI, RhlR is able to activate the inducible promoter of the rhl quorum sensing system of Pseudomonas aeruginosa.

RhlR binds to specific sequences in the promoter region and activates gene expression in the presence of N-butyryl-HSL [Medina et al., 2003].

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 7
Illegal NheI site found at 30
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 302
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 777

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K1073014 short</partinfo>
-The RhlR synthetase is constitutively expressed by a strong promoter.
+This device consists of a strong constitutive promoter (<partinfo>BBa_J23100</partinfo>) combined with RBS (<partinfo>BBa_B0032</partinfo>) and the coding region of the transcription regulator RhlR (<partinfo>BBa_C0071</partinfo>). This results in a constitutively high transcription level of RhlR.
-<!-- Add more about the biology of this part here
 ===Usage and Biology===
+In combination with N-butyryl homoserine lactone (N-butyryl-HSL) synthesized by RhlI, RhlR is able to activate the inducible promoter of the rhl quorum sensing system of ''Pseudomonas aeruginosa''.
+RhlR binds to specific sequences in the promoter region and activates gene expression in the presence of N-butyryl-HSL [Medina et al., 2003].
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