Difference between revisions of "Part:BBa K1073011"

Latest revision as of 08:56, 17 October 2013

Inducible ampicillin resistance cassette (regulated by RhlR + N-buturyl-HSL)

This device is a combination of BBa_K1073001 and BBa_K1073000 and mediates an inducible ampicillin resistance. It includes the inducible promoter of the rhl quorum sensing system of Pseudomonas aeruginosa followed by a RBS and the ampicillin resistance gene coding for beta-lactamase.

Usage and Biology

The promoter is activated in the presence of RhlR in combination with the autoinducer molecule N-butyryl-homoserine lactone produced by RhlI. The expression of the beta-lactamase can therefore be induced and tuned by supplying RhlR and N-butyryl-HSL.

Note that the promoter is leaking to a certain degree when not activated. For further information and experience on this device see BBa_K1073035.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 5: / Line 5: @@
 ===Usage and Biology===
-The promoter is activated in the presence of RhlR in combination with the autoinducer molecule N-buturyl-homoserine lactone produced by RhlI. The expression of the beta-lactamase can therefore be induced and tuned by supplying RhlR and N-buturyl-HSL.
+The promoter is activated in the presence of RhlR in combination with the autoinducer molecule N-butyryl-homoserine lactone produced by RhlI. The expression of the beta-lactamase can therefore be induced and tuned by supplying RhlR and N-butyryl-HSL.
 Note that the promoter is leaking to a certain degree when not activated. For further information and experience on this device see <partinfo>BBa_K1073035</partinfo>.