Difference between revisions of "Part:BBa K1155003"
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<partinfo>BBa_K1155003 short</partinfo> | <partinfo>BBa_K1155003 short</partinfo> | ||
| − | AmilCP is a blue/purple chromoprotein from coral ''Acropora millepora''. This protein exhibits a strong colour that | + | AmilCP is a blue/purple chromoprotein from coral ''Acropora millepora''. This protein exhibits a strong colour that can visualized by naked eyes. The biobrick part (BBa_K592009) consisting of the amilCP gene was created by Team Uppsala - Sweden 2011. |
| − | In order to be easily used by all the other teams, our team improved the biobrick BBa_K592009 by assembly of biobricks B0034, BBa_K592009 and B0015 to construct a composite part including a ribosome binding site, full length of amilCP gene and double terminator. We used it as one of our reporter gene in ''Escherichia coli''. | + | In order to be easily used by all the other teams, our team improved the biobrick [https://parts.igem.org/Part:BBa_K592009 BBa_K592009] by assembly of biobricks B0034, BBa_K592009 and B0015 to construct a composite part including a ribosome binding site, full length of amilCP gene and double terminator. We used it as one of our reporter gene in ''Escherichia coli''. |
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<partinfo>BBa_K1155003 parameters</partinfo> | <partinfo>BBa_K1155003 parameters</partinfo> | ||
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| − | '''Characterization :''' | + | ==='''Characterization :'''=== |
We have tested the Biobricks BBa_K1155003 in the construction: BBa_K1155000 + BBa_K1155003 in pSB1C3 | We have tested the Biobricks BBa_K1155003 in the construction: BBa_K1155000 + BBa_K1155003 in pSB1C3 | ||
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- O2 : --> no color | - O2 : --> no color | ||
| − | [[File:PsPfnr3008.jpg]] | + | [[File:PsPfnr3008.jpg|500px]] |
These results indicate that the composite part BBa_K1155003 composed of a RBS + amilCP + terminator works very well and can be easily used as a reporter system of promoteur expression. | These results indicate that the composite part BBa_K1155003 composed of a RBS + amilCP + terminator works very well and can be easily used as a reporter system of promoteur expression. | ||
Latest revision as of 07:21, 5 October 2013
RBS-amilCP-term
AmilCP is a blue/purple chromoprotein from coral Acropora millepora. This protein exhibits a strong colour that can visualized by naked eyes. The biobrick part (BBa_K592009) consisting of the amilCP gene was created by Team Uppsala - Sweden 2011.
In order to be easily used by all the other teams, our team improved the biobrick BBa_K592009 by assembly of biobricks B0034, BBa_K592009 and B0015 to construct a composite part including a ribosome binding site, full length of amilCP gene and double terminator. We used it as one of our reporter gene in Escherichia coli.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Characterization :
We have tested the Biobricks BBa_K1155003 in the construction: BBa_K1155000 + BBa_K1155003 in pSB1C3
BBa_K1155000 is a promoteur Pndh* repressed in anaerobic condition by binding of the FNR regulator.(see BBa_K1155000 for more information)
What we expected :
+ O2 : FNR inactivated : Pndh* activated --> amilCP expression --> purple color
- O2 : FNR activated : Pndh* repressed --> amilCP not expressed --> no color
What we observed :
+ O2 : --> purple color
- O2 : --> no color
These results indicate that the composite part BBa_K1155003 composed of a RBS + amilCP + terminator works very well and can be easily used as a reporter system of promoteur expression.