Difference between revisions of "Part:BBa K1155003"
 
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<partinfo>BBa_K1155003 short</partinfo>
 
<partinfo>BBa_K1155003 short</partinfo>
  
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Improvement of the biobrick BBa_K592009 designed by iGEM11_Uppsala-Sweden in order to be easily used by all the other teams.
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AmilCP is a blue/purple chromoprotein from coral ''Acropora millepora''. This protein exhibits a strong colour that can visualized by naked eyes. The biobrick part (BBa_K592009) consisting of the amilCP gene was created by Team Uppsala - Sweden 2011.  
  
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assembly of biobricks B0034, BBa_K592009 and B0015
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In order to be easily used by all the other teams, our team improved the biobrick [https://parts.igem.org/Part:BBa_K592009 BBa_K592009] by assembly of biobricks B0034, BBa_K592009 and B0015 to construct a composite part including a ribosome binding site, full length of amilCP gene and double terminator. We used it as one of our reporter gene in ''Escherichia coli''.
  
 
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<partinfo>BBa_K1155003 parameters</partinfo>
 
<partinfo>BBa_K1155003 parameters</partinfo>
 
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==='''Characterization :'''===
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We have tested the Biobricks BBa_K1155003 in the construction: BBa_K1155000 + BBa_K1155003 in pSB1C3
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BBa_K1155000 is a promoteur Pndh* repressed in anaerobic condition by binding of the FNR regulator.(see BBa_K1155000 for more information)
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What we expected :
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+ O2 : FNR inactivated : Pndh* activated --> amilCP expression --> purple color
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- O2 : FNR activated : Pndh* repressed --> amilCP not expressed --> no color
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What we observed :
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+ O2 : --> purple color
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- O2 : --> no color
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[[File:PsPfnr3008.jpg|500px]]
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These results indicate that the composite part BBa_K1155003 composed of a RBS + amilCP + terminator works very well and can be easily used as a reporter system of promoteur expression.

Latest revision as of 07:21, 5 October 2013

RBS-amilCP-term

AmilCP is a blue/purple chromoprotein from coral Acropora millepora. This protein exhibits a strong colour that can visualized by naked eyes. The biobrick part (BBa_K592009) consisting of the amilCP gene was created by Team Uppsala - Sweden 2011.

In order to be easily used by all the other teams, our team improved the biobrick BBa_K592009 by assembly of biobricks B0034, BBa_K592009 and B0015 to construct a composite part including a ribosome binding site, full length of amilCP gene and double terminator. We used it as one of our reporter gene in Escherichia coli.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Characterization :

We have tested the Biobricks BBa_K1155003 in the construction: BBa_K1155000 + BBa_K1155003 in pSB1C3

BBa_K1155000 is a promoteur Pndh* repressed in anaerobic condition by binding of the FNR regulator.(see BBa_K1155000 for more information)

What we expected :

+ O2 : FNR inactivated : Pndh* activated --> amilCP expression --> purple color

- O2 : FNR activated : Pndh* repressed --> amilCP not expressed --> no color

What we observed :

+ O2 : --> purple color

- O2 : --> no color

PsPfnr3008.jpg

These results indicate that the composite part BBa_K1155003 composed of a RBS + amilCP + terminator works very well and can be easily used as a reporter system of promoteur expression.