Difference between revisions of "Part:BBa K1150011"

 
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K1150011 short</partinfo>
 
<partinfo>BBa_K1150011 short</partinfo>
  
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{| style="color:black" cellpadding="6" cellspacing="1" border="2" align="right"
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! colspan="2" style="background:#FFBF00;"|SV40
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|-
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|'''Function'''
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|Promoter
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|-
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|'''Use in'''
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|Mammalian cells
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|-
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|'''RFC standard'''
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|[https://parts.igem.org/Help:Assembly_standard_10 RFC 10]
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|-
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|'''Backbone'''
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|pSB1C3<br>
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|-
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|'''Submitted by'''
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|[http://2013.igem.org/Team:Freiburg Freiburg 2013]
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|}
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SV40 promoter is a viral promoter derived from Simian Virus 40.
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Because we could not find the SV40 promotor in the parts registry, the Freiburg iGEM Team 2013 decided to provide one to the following teams. This enables the teams to choose from differently strong mammalian promoters – dependent of the individual intention.
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We tested our own devices with both promoters ([https://parts.igem.org/Part:BBa_K1150011 BBa_K1150011] [SV40], [https://parts.igem.org/Part:BBa_K747096 BBa_K747096] [CMV]). For further information please take a look at [https://parts.igem.org/Part:BBa_K1150019 BBa_K1150019] and [https://parts.igem.org/Part:BBa_K1150020 BBa_K1150020] or click [http://2013.igem.org/Team:Freiburg/Project/effector#activation here].
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In an earlier study CMV and SV40 promoter were tested in different B and T cells as well as in two non-lymphoid cells. In all cell lines the CMV promoter construct was higher expressed than that of SV40 [Zarrin ''et al.'', 1999].
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In comparison to the cellular promoter VV1 SV40 showed similar activities. Hence with an SV40 promoter one can gain a more physiologic control for reporter assays compared to CMV.
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<span class='h3bb'>Sequence and Features</span>
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<span class='h3bb'>
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==Sequence and Features==
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</span>
 
<partinfo>BBa_K1150011 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K1150011 SequenceAndFeatures</partinfo>
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==References==
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<small>
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ZARRIN, A.; MALKIN, L.; FONG, I.; LUK, K.; GHOSE, A.; BERINSTEIN, N. (1999). Comparison of CMV, RSV, SV40 viral and VV1 cellular promoters in B and T lymphoid and non-lymphoid cell lines. ''Biochimica et Biophysica Acta'' 1446: 135-139.
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</small>
  
  

Latest revision as of 21:33, 4 October 2013

SV40 promoter

SV40
Function Promoter
Use in Mammalian cells
RFC standard RFC 10
Backbone pSB1C3
Submitted by [http://2013.igem.org/Team:Freiburg Freiburg 2013]


SV40 promoter is a viral promoter derived from Simian Virus 40.

Because we could not find the SV40 promotor in the parts registry, the Freiburg iGEM Team 2013 decided to provide one to the following teams. This enables the teams to choose from differently strong mammalian promoters – dependent of the individual intention. We tested our own devices with both promoters (BBa_K1150011 [SV40], BBa_K747096 [CMV]). For further information please take a look at BBa_K1150019 and BBa_K1150020 or click [http://2013.igem.org/Team:Freiburg/Project/effector#activation here].

In an earlier study CMV and SV40 promoter were tested in different B and T cells as well as in two non-lymphoid cells. In all cell lines the CMV promoter construct was higher expressed than that of SV40 [Zarrin et al., 1999].

In comparison to the cellular promoter VV1 SV40 showed similar activities. Hence with an SV40 promoter one can gain a more physiologic control for reporter assays compared to CMV.



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

References

ZARRIN, A.; MALKIN, L.; FONG, I.; LUK, K.; GHOSE, A.; BERINSTEIN, N. (1999). Comparison of CMV, RSV, SV40 viral and VV1 cellular promoters in B and T lymphoid and non-lymphoid cell lines. Biochimica et Biophysica Acta 1446: 135-139.