Difference between revisions of "Part:BBa K1067007"

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<partinfo>BBa_K1067007 short</partinfo>
 
<partinfo>BBa_K1067007 short</partinfo>
  
No leaky pBAD promoter with araC constitutively expressed. This biobrick is the same as [[part:BBa_K808000]] but with a change i pBAD promoter and another sequence annotation. The promoter is taken from the [[pBAD SPL]] from DTU iGEM team 2013.
+
Non leaky pBAD promoter with araC constitutively expressed. This biobrick is the same as [[part:BBa_K808000]] but with a change i pBAD promoter and another sequence annotation. The promoter is taken from the [[pBAD SPL]] from DTU iGEM team 2013.
  
  

Revision as of 15:55, 4 October 2013

Tight pBAD with araC

Non leaky pBAD promoter with araC constitutively expressed. This biobrick is the same as part:BBa_K808000 but with a change i pBAD promoter and another sequence annotation. The promoter is taken from the pBAD SPL from DTU iGEM team 2013.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 1182
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 1182
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 1182
    Illegal AgeI site found at 979
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961