Difference between revisions of "PBAD SPL"
| Line 7: | Line 7: | ||
! style="background:#AAAAAA; color:black" |Identifier | ! style="background:#AAAAAA; color:black" |Identifier | ||
! style="background:#AAAAAA; color:black" |Sequence<sup>a</sup> | ! style="background:#AAAAAA; color:black" |Sequence<sup>a</sup> | ||
| − | ! colspan="2" style="background:#AAAAAA; color:black" | | + | ! colspan="2" style="background:#AAAAAA; color:black" |Induced Strength<sup>b</sup> |
| + | ! colspan="2" style="background:#AAAAAA; color:black" |Leakyness<sup>b</sup> | ||
|- | |- | ||
|align="center"|Col. 2 | |align="center"|Col. 2 | ||
|<font face="Courier" size="+1">CTGACGACGCCCCTCTCCGCCCCTTAAAATGTCCA</font> | |<font face="Courier" size="+1">CTGACGACGCCCCTCTCCGCCCCTTAAAATGTCCA</font> | ||
| + | |align="center"|14.2 | ||
|align="center"|14.2 | |align="center"|14.2 | ||
|- | |- | ||
| Line 16: | Line 18: | ||
|<font face="Courier" size="+1">CTGACGTAATCATACCGCCGAAAGTATTATCATTA</font> | |<font face="Courier" size="+1">CTGACGTAATCATACCGCCGAAAGTATTATCATTA</font> | ||
|align="center"|16.6 | |align="center"|16.6 | ||
| + | |align="center"|14.2 | ||
|- | |- | ||
|align="center"|Col. 4 | |align="center"|Col. 4 | ||
|<font face="Courier" size="+1">CTGACGCGACAACATTGCGTCCTATAAAATGCCGA</font> | |<font face="Courier" size="+1">CTGACGCGACAACATTGCGTCCTATAAAATGCCGA</font> | ||
| + | |align="center"|14.2 | ||
|align="center"|14.2 | |align="center"|14.2 | ||
|- | |- | ||
Revision as of 10:30, 4 October 2013
Here the DTU iGEM team will present their work on characterizing a SPL of pBAD promoters.
List of sequences:
DTU pBAD SPL
| Identifier | Sequencea | Induced Strengthb | Leakynessb | ||
|---|---|---|---|---|---|
| Col. 2 | CTGACGACGCCCCTCTCCGCCCCTTAAAATGTCCA | 14.2 | 14.2 | ||
| Col. 3 | CTGACGTAATCATACCGCCGAAAGTATTATCATTA | 16.6 | 14.2 | ||
| Col. 4 | CTGACGCGACAACATTGCGTCCTATAAAATGCCGA | 14.2 | 14.2 | ||
| BBa_J23102 | ttgacagctagctcagtcctaggtactgtgctagc | 0.86 | |||
| BBa_J23103 | ctgatagctagctcagtcctagggattatgctagc | 0.01 | |||
| BBa_J23104 | ttgacagctagctcagtcctaggtattgtgctagc | 0.72 | |||
| BBa_J23105 | tttacggctagctcagtcctaggtactatgctagc | 0.24 | |||
| BBa_J23106 | tttacggctagctcagtcctaggtatagtgctagc | 0.47 | |||
| BBa_J23107 | tttacggctagctcagccctaggtattatgctagc | 0.36 | |||
| BBa_J23108 | ctgacagctagctcagtcctaggtataatgctagc | 0.51 | |||
| BBa_J23109 | tttacagctagctcagtcctagggactgtgctagc | 0.04 | |||
| BBa_J23110 | tttacggctagctcagtcctaggtacaatgctagc | 0.33 | |||
| BBa_J23111 | ttgacggctagctcagtcctaggtatagtgctagc | 0.58 | |||
| BBa_J23112 | ctgatagctagctcagtcctagggattatgctagc | 0.00 | |||
| BBa_J23113 | ctgatggctagctcagtcctagggattatgctagc | 0.01 | |||
| BBa_J23114 | tttatggctagctcagtcctaggtacaatgctagc | 0.10 | |||
| BBa_J23115 | tttatagctagctcagcccttggtacaatgctagc | 0.15 | |||
| BBa_J23116 | ttgacagctagctcagtcctagggactatgctagc | 0.16 | |||
| BBa_J23117 | ttgacagctagctcagtcctagggattgtgctagc | 0.06 | |||
| BBa_J23118 | ttgacggctagctcagtcctaggtattgtgctagc | 0.56 | |||
| Mutant name | Sequence |
|---|---|
| Mut. 1 | CTGACGACGCCCCTCTCCGCCCCTTAAAATGTCCA |
| Mut. 2 | CTGACGTAATCATACCGCCGAAAGTATTATCATTA |
| Mut. 3 | CTGACGCGACAACATTGCGTCCTATAAAATGCCGA |