Difference between revisions of "Part:BBa K1127016"

Latest revision as of 14:56, 3 October 2013

Gold and QS ultra complex

The device is a tunable hybrid bistable switch. It receives two signals 1. gold (HAuCl4) and 2. population density, which modify magnitude of the output 'transactivation of the target gene'.

LuxR [1] is produced constitutively by the promoter BBa_J23100 [2]. It is activated by the quorum sensing signal AHL and forms a feedback loop to promote expression of the AHL synthase LuxI [3] and the transactivator taR12 [4]. The latter binds to crR12 [5] preceding translation start site of the mRNA of the target gene resulting in full expression. Gold is recognized by the part BBa_K1127008 [6] which in response produces AiiA [7]. AiiA can degrade AHL; therefore, it reduces expression of the target gene. The mechanism is simplified and shown in Figure 1.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 1747
Illegal NheI site found at 1770
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 3565
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 701
Illegal AgeI site found at 880
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 2808

@@ Line 4: / Line 4: @@
 The device is a tunable hybrid bistable switch. It receives two signals 1. gold (HAuCl4) and 2. population density, which modify magnitude of the output 'transactivation of the target gene'.
-Gold is recognized by the part BBa_K1127008 [https://parts.igem.org/wiki/index.php?title=Part:BBa_K1127008] which in response produces AiiA [https://parts.igem.org/Part:BBa_K516022]. AiiA can degrade the quorum sensing signal AHL; therefore, it reduces expression of the target gene.
+LuxR [https://parts.igem.org/Part:BBa_I0462] is produced constitutively by the promoter BBa_J23100 [https://parts.igem.org/Part:BBa_J23100]. It is activated by the quorum sensing signal AHL and forms a feedback loop to promote expression of the AHL synthase LuxI [https://parts.igem.org/Part:BBa_K805016] and the transactivator taR12 [https://parts.igem.org/Part:BBa_J01008]. The latter binds to crR12 [https://parts.igem.org/Part:BBa_J01010] preceding translation start site of the mRNA of the target gene resulting in full expression.
-LuxR [https://parts.igem.org/Part:BBa_I0462] is produced constitutively by the promoter BBa_J23100 [https://parts.igem.org/Part:BBa_J23100]. It is activated by AHL and forms a positive feedback loop to promote expression of the AHL synthases LuxI [https://parts.igem.org/Part:BBa_K805016] and transactivating RNA taR12 [https://parts.igem.org/Part:BBa_J01008].
+Gold is recognized by the part BBa_K1127008 [https://parts.igem.org/wiki/index.php?title=Part:BBa_K1127008] which in response produces AiiA [https://parts.igem.org/Part:BBa_K516022]. AiiA can degrade AHL; therefore, it reduces expression of the target gene. The mechanism is simplified and shown in Figure 1.
+[[File:The hybrid bistable switch.jpg|400px|thumb|center]]
 <!-- Add more about the biology of this part here