Difference between revisions of "Part:BBa K1065308"
 
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<partinfo>BBa_K1065308 short</partinfo>
 
<partinfo>BBa_K1065308 short</partinfo>
  
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This is an intermediate part to build a blue light inducible genetic circuit. The part produces YF1 and FixJ under the control of a strong promoter of the Anderson family and the inverter <partinfo>BBa_S04617</partinfo>.
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This is an intermediate part to build a blue light inducible genetic circuit. The part produces YF1 and FixJ under the control of a strong promoter of the Anderson family <partinfo>BBa:J23100</partinfo>. It is present also an inverter cassette regulated by pFixK2: it contains the inverter cI and the inducible promoter pLambda.
 
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This part was used to create a complete blue light circuit <partinfo>BBa_K1065310</partinfo> that was fully characterized by iGEM UniTN Trento 2013.
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Note that this part is in pSB1A2 and it was not submitted to the registry. If you are interested in this part please contact iGEM UniTN Trento 2013.  
  
 
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Latest revision as of 09:49, 3 October 2013

J23100+YF1+FixJ+FixK2+inverter

This is an intermediate part to build a blue light inducible genetic circuit. The part produces YF1 and FixJ under the control of a strong promoter of the Anderson family BBa_J23100. It is present also an inverter cassette regulated by pFixK2: it contains the inverter cI and the inducible promoter pLambda.

Note that this part is in pSB1A2 and it was not submitted to the registry. If you are interested in this part please contact iGEM UniTN Trento 2013.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 605
    Illegal NgoMIV site found at 677
    Illegal NgoMIV site found at 767
    Illegal NgoMIV site found at 785
    Illegal NgoMIV site found at 1297
    Illegal NgoMIV site found at 1590
    Illegal NgoMIV site found at 1684
    Illegal AgeI site found at 319
    Illegal AgeI site found at 1465
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1354
    Illegal BsaI.rc site found at 218