Difference between revisions of "Part:BBa K1127016"

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The device is a tunable hybrid bistable switch. It receives two signals 1. gold (HAuCl4) and 2. population density, which modify magnitude of the output 'transactivation of the target gene'.
 
The device is a tunable hybrid bistable switch. It receives two signals 1. gold (HAuCl4) and 2. population density, which modify magnitude of the output 'transactivation of the target gene'.
  
LuxR [https://parts.igem.org/Part:BBa_I0462] is produced constitutively by the promoter BBa_J23100 [https://parts.igem.org/Part:BBa_J23100]. It is activated by the quorum sensing signal AHL and forms a positive feedback loop to promote expression of the AHL synthase LuxI [https://parts.igem.org/Part:BBa_K805016] and transactivating RNA taR12 [https://parts.igem.org/Part:BBa_J01008].  
+
LuxR [https://parts.igem.org/Part:BBa_I0462] is produced constitutively by the promoter BBa_J23100 [https://parts.igem.org/Part:BBa_J23100]. It is activated by the quorum sensing signal AHL and forms a positive feedback loop to promote expression of the AHL synthase LuxI [https://parts.igem.org/Part:BBa_K805016] and the activator taR12 [https://parts.igem.org/Part:BBa_J01008]. The latter binds to crR12 [https://parts.igem.org/Part:BBa_J01010] preceding translation start site of the mRNA of the target gene resulting in the full expression.
 
Gold is recognized by the part BBa_K1127008 [https://parts.igem.org/wiki/index.php?title=Part:BBa_K1127008] which in response produces AiiA [https://parts.igem.org/Part:BBa_K516022]. AiiA can degrade AHL; therefore, it reduces expression of the target gene.  
 
Gold is recognized by the part BBa_K1127008 [https://parts.igem.org/wiki/index.php?title=Part:BBa_K1127008] which in response produces AiiA [https://parts.igem.org/Part:BBa_K516022]. AiiA can degrade AHL; therefore, it reduces expression of the target gene.  
  

Revision as of 21:08, 29 September 2013

Gold and QS ultra complex

The device is a tunable hybrid bistable switch. It receives two signals 1. gold (HAuCl4) and 2. population density, which modify magnitude of the output 'transactivation of the target gene'.

LuxR [1] is produced constitutively by the promoter BBa_J23100 [2]. It is activated by the quorum sensing signal AHL and forms a positive feedback loop to promote expression of the AHL synthase LuxI [3] and the activator taR12 [4]. The latter binds to crR12 [5] preceding translation start site of the mRNA of the target gene resulting in the full expression. Gold is recognized by the part BBa_K1127008 [6] which in response produces AiiA [7]. AiiA can degrade AHL; therefore, it reduces expression of the target gene.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1747
    Illegal NheI site found at 1770
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 3565
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 701
    Illegal AgeI site found at 880
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2808