Difference between revisions of "Part:BBa K1073024:Experience"
(→User Reviews) |
(→User Reviews) |
||
Line 27: | Line 27: | ||
[[File:BS2013Emission amilGFP.jpg |400px]] | [[File:BS2013Emission amilGFP.jpg |400px]] | ||
− | '''iGEM Team Braunschweig 2013:''' Emission spectrum of chromoprotein amilGFP. This chromoprotein does show significant emission | + | '''iGEM Team Braunschweig 2013:''' Emission spectrum of chromoprotein amilGFP. This chromoprotein does show significant emission that can be detected by its fluorescence. |
'''Emission maximum of amilGFP:''' 515,8 nm | '''Emission maximum of amilGFP:''' 515,8 nm |
Revision as of 11:49, 28 September 2013
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_K1073024
When amilGFP is expressed, the colonies on plates as well as the liquid culture become yellow.
User Reviews
UNIQ3a60aeef05c451c3-partinfo-00000000-QINU
•••••
iGEM Team Braunschweig 2013 |
When expressed the chromoprotein amilGFP exhibits a strong yellow color. In order to avoid absorption by the chomoproteins during the measurement of the OD of bacterial cultures, the absorption and emission spectra were measured and an optimal wavelength for spectral optical density measurement was determined to be at 520nm. iGEM Team Braunschweig 2013: Absorption spectrum of chromoprotein amilGFP in the wavelength range 400-800 nm. Absorption maximum of amilGFP: 504 nm iGEM Team Braunschweig 2013: Emission spectrum of chromoprotein amilGFP. This chromoprotein does show significant emission that can be detected by its fluorescence. Emission maximum of amilGFP: 515,8 nm
Although this device is working perfectly the way it is expected to the iGEM Team Braunschweig 2013 did not use it because the yellow color is not as easiely distinguishable by naked eye from the color of the media as the other chromoproteins are. iGEM Team Braunschweig 2013: Supernatant after cell disruption containing chromoproteins.
|
UNIQ3a60aeef05c451c3-partinfo-00000004-QINU