Difference between revisions of "Part:BBa K1073016"

Latest revision as of 11:19, 28 September 2013

Constitutively expressed transcription regulator LasR + LVA

The device consists of a strong constitutive promoter (BBa_J23100) combined with RBS (BBa_B0032) and the encoding region of the transcription regulator LasR (BBa_C0079).This results in a constitutively high transcription level of LasR.

Usage and Biology

In combination with N-3-oxo-dodecanoyl-HSL synthesized by LasI, LasR is able to activate the inducible promoter of the las quorum sensing system of Pseudomonas aeruginosa.

LasR binds to specific sequences in the promoter region. In the presence of N-3-oxo-dodecanoyl-HSL it activates gene expression (Medina et al., 2003).

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 7
Illegal NheI site found at 30
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 384
Illegal AgeI site found at 581
1000
COMPATIBLE WITH RFC[1000]

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K1073016 short</partinfo>
-The LasR activator is constitutively expressed by a strong promoter. It accepts the chemical signal AI-1 (made by lasI protein)
+The device consists of a strong constitutive promoter (<partinfo>BBa_J23100</partinfo>) combined with RBS (<partinfo>BBa_B0032</partinfo>) and the encoding region of the transcription regulator LasR (<partinfo>BBa_C0079</partinfo>).This results in a constitutively high transcription level of LasR.
-<!-- Add more about the biology of this part here
 ===Usage and Biology===
+In combination with N-3-oxo-dodecanoyl-HSL synthesized by LasI, LasR is able to activate the inducible promoter of the las quorum sensing system of ''Pseudomonas aeruginosa''.
+LasR binds to specific sequences in the promoter region. In the presence of N-3-oxo-dodecanoyl-HSL it activates gene expression (Medina et al., 2003).
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