Difference between revisions of "Part:BBa K1073020:Experience"

iGEM Team Braunschweig 2013

The chromoprotein aeBlue exhibits a distinct blue color when expressed. In order to avoid absorption of the chomoproteins during OD measurement of a bacterial culture, the absorption and emission spectra were measured and an optimal wavelength for spectral optical density measurement was determined according to these spectra.

iGEM Team Braunschweig 2013: Absorption spectrum of chromoprotein aeBlue in a wavelength range of 400-800 nm.

Absorption maximum of aeBlue: 595 nm

iGEM Team Braunschweig 2013:Emission spectrum of chromoprotein aeBlue. This chromoprotein does not show any significant emission in a wavelength range between 500-800 nm and therefore cannot be detected by its fluorescence.

Emission maximum of aeBlue: none (dark quencher)

AeBlue is a so called dark quencher protein. When excited this kind of chromophores relax back to the ground state without emitting energy through radiation and remain dark [Le Reste et al., 2012].

According to these results the optical density for growth curves of cells expressing this protein was measured at 520 nm with respect to the chromoproteins amilGFP (BBa_K1073024) and eforRed (BBa_K1073022) to avoid absorption by the proteins. Comparing all absorption spectra they all show an absorption minimum at 520 nm.