Difference between revisions of "Part:BBa K1162006"
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===Improvement on Existing Parts (as of September 2013)=== | ===Improvement on Existing Parts (as of September 2013)=== | ||
| − | [https://parts.igem.org/wiki/index.php?title=Part:BBa_K245114 BBa_K245114] (Slovenia iGEM team 2009) - This part lacks a start codon. The BBa_K1162006 part improves upon this one by adding a Met(atg) residue at the beginning of the | + | [https://parts.igem.org/wiki/index.php?title=Part:BBa_K245114 BBa_K245114] (Slovenia iGEM team 2009) - This part lacks a start codon, which would require additional design to be expressed individually. The BBa_K1162006 part improves upon this one by adding a Met(atg) residue at the beginning of the coding region. It is unclear if Slovenia's part has been codon optimized for <i>E. coli</i>, but our new part has been designed with this in mind by Life Technologies (see design). |
| − | [https://parts.igem.org/Part:BBa_K875009?title=Part:BBa_K875009 BBa_K875009] (Trieste iGEM team 2012) - This part, BBa_K875009, | + | [https://parts.igem.org/Part:BBa_K875009?title=Part:BBa_K875009 BBa_K875009] (Trieste iGEM team 2012) - This part, BBa_K875009, uses the [https://parts.igem.org/Assembly_standard_10 Assembly Standard #10] for both the prefix and suffix and therefore cannot be used for protein fusions, such as in cases of secretion, protein purification, or fusion proteins. This part also has a stop codon which further limits what can be added to the C-terminal end (purification tag/secretion tag). The BBa_K1162006 improves upon this part by using [https://parts.igem.org/Assembly_standard_23 Assembly Standard #23 (Silver Fusion)] and by removing the stop codon at the end of the coding region. Additionally, the BBa_K1162006 part was codon optimized by Life Technologies (see design). |
===References=== | ===References=== | ||
Revision as of 02:24, 28 September 2013
LL-37 antimicrobial peptide from Humans (Homo sapiens)
LL-37 is an antimicrobial peptide (AMP) found in humans.
Design
This part was codon optimized for E. coli using the Life Technologies GeneArt ® software program. By design, this part is Assembly Standard #23 (Silver Fusion) and contains a Methionine(atg) residue at the start of the coding region. Furthermore, this LL-37 sequence does not have any stop codons, which allows for composite BioBrick construction.
Improvement on Existing Parts (as of September 2013)
BBa_K245114 (Slovenia iGEM team 2009) - This part lacks a start codon, which would require additional design to be expressed individually. The BBa_K1162006 part improves upon this one by adding a Met(atg) residue at the beginning of the coding region. It is unclear if Slovenia's part has been codon optimized for E. coli, but our new part has been designed with this in mind by Life Technologies (see design).
BBa_K875009 (Trieste iGEM team 2012) - This part, BBa_K875009, uses the Assembly Standard #10 for both the prefix and suffix and therefore cannot be used for protein fusions, such as in cases of secretion, protein purification, or fusion proteins. This part also has a stop codon which further limits what can be added to the C-terminal end (purification tag/secretion tag). The BBa_K1162006 improves upon this part by using Assembly Standard #23 (Silver Fusion) and by removing the stop codon at the end of the coding region. Additionally, the BBa_K1162006 part was codon optimized by Life Technologies (see design).
References
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]