Difference between revisions of "Part:BBa K1189018"

Revision as of 01:37, 28 September 2013

Human ferritin di-subunit with E coil w/ LacI promoter

This part was created by fusing the heavy chain and light chains (BBa_K1189024 BBa_K1189025) of human ferritin together. It is expressed under the lacI promoter (BBa_J04500) and has a his-tag for protein purification. An E-coil (BBa_K1189011) is included in order to allow binding of parts containing the respective K-coil (BBa_K1189010).

This construct can be used as a reporter through a modification of the iron core to form Prussian Blue (Figure 1). The resulting molecule can then catalyze the formation of radicals from hydrogen peroxide, which can then cause a colour change in substrates such as TMB or ABTS (Figure 2).

Figure 1. Comparison image of commercial ferritin to Prussian blue ferritin after the synthesis reaction. The synthesis reaction took place over a 12 hour time period.

Figure 2. Image of the colours of ABTS and TMB (10 mg/mL for both) after reacting with Prussian blue ferritin.

Sequence and Features