Difference between revisions of "Part:BBa K1031742"
 
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<partinfo>BBa_K1031742 short</partinfo>
 
<partinfo>BBa_K1031742 short</partinfo>
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<p>For detailed information concerning PaaX, please visit <a href="http://2013.igem.org/Team:Peking/Project/BioSensors/HcaR#Mileston3">2013 Peking iGEM Biosensor PaaX</a></p>
  
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<img src="https://static.igem.org/mediawiki/igem.org/c/c9/Peking_Logo.jpg" style="width:960px;"/>
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== '''Construction''' ==
 
== '''Construction''' ==
  
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In order to fine-tune the performance of PaaX biosensor, we constructed a library of ''Pc'' constitutive promoters at different intensity. BBa_K1031742 is composed of three elements, the constitutive ''Pc'' promoter J23109[https://parts.igem.org/Part:BBa_J23109], coding sequence of PaaX and terminator B0015[https://parts.igem.org/Part:BBa_B0015]. ('''Fig 1''')
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In order to fine-tune the performance of PaaX biosensor, we constructed a library of ''Pc'' constitutive promoters at different intensity. BBa_K1031742 is composed of three elements, the constitutive ''Pc'' promoter <html><a href="https://parts.igem.org/Part:BBa_J23109">J23109</a></html>, coding sequence of PaaX and terminator <html><a href="https://parts.igem.org/Part:BBa_B0015">B0015</a></html>. ('''Fig 1''')
  
 
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<img src="https://static.igem.org/mediawiki/igem.org/thumb/f/fe/Peking2013_part_109-PaaX-TT.png/800px-Peking2013_part_109-PaaX-TT.png", width=600px; />
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<img src="https://static.igem.org/mediawiki/igem.org/thumb/f/fe/Peking2013_part_109-PaaX-TT.png/800px-Peking2013_part_109-PaaX-TT.png" style="width:600px; margin-left:170px" />
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<p style="text-align:center"><b>Fig 1</b> Construction of biosensor circuit. Orange arrowheads represent promoters. RBS is shown as green oval and square in dark red stands for B0015 terminator.
 
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'''Fig 1''' Construction of biosensor circuit. Orange arrowheads represent promoters. RBS is shown as green oval and square in dark red stands for B0015 terminator.
 
  
  

Latest revision as of 22:40, 27 September 2013

J23109-PaaX

For detailed information concerning PaaX, please visit 2013 Peking iGEM Biosensor PaaX


Construction

In order to fine-tune the performance of PaaX biosensor, we constructed a library of Pc constitutive promoters at different intensity. BBa_K1031742 is composed of three elements, the constitutive Pc promoter J23109, coding sequence of PaaX and terminator B0015. (Fig 1)

Fig 1 Construction of biosensor circuit. Orange arrowheads represent promoters. RBS is shown as green oval and square in dark red stands for B0015 terminator.



Sequence and Features

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 147
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 344
    Illegal NgoMIV site found at 625
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 851
    Illegal SapI.rc site found at 1508