Difference between revisions of "Part:BBa K1031742"
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__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_K1031742 short</partinfo> | <partinfo>BBa_K1031742 short</partinfo> | ||
+ | <html> | ||
+ | <p>For detailed information concerning PaaX, please visit <a href="http://2013.igem.org/Team:Peking/Project/BioSensors/HcaR#Mileston3">2013 Peking iGEM Biosensor PaaX</a></p> | ||
− | < | + | <img src="https://static.igem.org/mediawiki/igem.org/c/c9/Peking_Logo.jpg" style="width:960px;"/> |
+ | </html> | ||
== '''Construction''' == | == '''Construction''' == | ||
− | + | In order to fine-tune the performance of PaaX biosensor, we constructed a library of ''Pc'' constitutive promoters at different intensity. BBa_K1031742 is composed of three elements, the constitutive ''Pc'' promoter <html><a href="https://parts.igem.org/Part:BBa_J23109">J23109</a></html>, coding sequence of PaaX and terminator <html><a href="https://parts.igem.org/Part:BBa_B0015">B0015</a></html>. ('''Fig 1''') | |
− | In order to fine-tune the performance of PaaX biosensor, we constructed a library of ''Pc'' constitutive promoters at different intensity. BBa_K1031742 is composed of three elements, the constitutive ''Pc'' promoter | + | |
<html> | <html> | ||
− | <img src="https://static.igem.org/mediawiki/igem.org/thumb/f/fe/Peking2013_part_109-PaaX-TT.png/800px-Peking2013_part_109-PaaX-TT.png" | + | <img src="https://static.igem.org/mediawiki/igem.org/thumb/f/fe/Peking2013_part_109-PaaX-TT.png/800px-Peking2013_part_109-PaaX-TT.png" style="width:600px; margin-left:170px" /> |
+ | <p style="text-align:center"><b>Fig 1</b> Construction of biosensor circuit. Orange arrowheads represent promoters. RBS is shown as green oval and square in dark red stands for B0015 terminator. | ||
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<!-- Uncomment this to enable Functional Parameter display | <!-- Uncomment this to enable Functional Parameter display | ||
===Functional Parameters=== | ===Functional Parameters=== | ||
− | <partinfo> | + | <partinfo>BBa_K1031742 parameters</partinfo> |
<!-- --> | <!-- --> |
Latest revision as of 22:40, 27 September 2013
J23109-PaaX
For detailed information concerning PaaX, please visit 2013 Peking iGEM Biosensor PaaX
Construction
In order to fine-tune the performance of PaaX biosensor, we constructed a library of Pc constitutive promoters at different intensity. BBa_K1031742 is composed of three elements, the constitutive Pc promoter J23109, coding sequence of PaaX and terminator B0015. (Fig 1)
Fig 1 Construction of biosensor circuit. Orange arrowheads represent promoters. RBS is shown as green oval and square in dark red stands for B0015 terminator.
Sequence and Features
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 147
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 344
Illegal NgoMIV site found at 625 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 851
Illegal SapI.rc site found at 1508