Difference between revisions of "Part:BBa K1031111"
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In order to fine-tune the performance of CapR biosensor, we constructed a library of ''Pc'' constitutive promoters at different intensity. BBa_K1031111 is composed of three elements, the constitutive ''Pc'' promoter J23109[https://parts.igem.org/Part:BBa_J23109], coding sequence of CapR and terminator B0015[https://parts.igem.org/Part:BBa_B0015]. ('''Fig 1''')
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In order to fine-tune the performance of CapR biosensor, we constructed a library of ''Pc'' constitutive promoters at different intensity. BBa_K1031111 is composed of three elements, the constitutive ''Pc'' promoter <html><a href="https://parts.igem.org/Part:BBa_J23109">J23109</a></html>, coding sequence of CapR and terminator <html><a href="https://parts.igem.org/Part:BBa_B0015">B0015</a></html>. ('''Fig 1''')
  
 
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<img src="https://static.igem.org/mediawiki/igem.org/thumb/2/2f/Peking2013_part_109-CapR-TT.png/800px-Peking2013_part_109-CapR-TT.png", width=600px; />
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<img src="https://static.igem.org/mediawiki/igem.org/thumb/2/2f/Peking2013_part_109-CapR-TT.png/800px-Peking2013_part_109-CapR-TT.png" style="width:600px; margin-left:170px" />
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<p style="text-align:center"><b>Fig 1</b> Construction of biosensor circuit ''Po''/J23109-CapR. The orange arrowheads represent promoters, RBS is shown as a green oval, and the red circle stands for B0015 terminator.
 
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'''Fig 1''' Construction of biosensor circuit ''Po''/J23109-CapR. The orange arrowheads represent promoters, RBS is shown as a green oval, and the red circle stands for B0015 terminator.
 
  
  

Revision as of 21:33, 27 September 2013

J23109-CapR-Terminator


Characterization

In order to fine-tune the performance of CapR biosensor, we constructed a library of Pc constitutive promoters at different intensity. BBa_K1031111 is composed of three elements, the constitutive Pc promoter J23109, coding sequence of CapR and terminator B0015. (Fig 1)

Fig 1 Construction of biosensor circuit ''Po''/J23109-CapR. The orange arrowheads represent promoters, RBS is shown as a green oval, and the red circle stands for B0015 terminator.


Sequence and Features

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 147
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 344
    Illegal NgoMIV site found at 625
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 851
    Illegal SapI.rc site found at 1508