Difference between revisions of "Part:BBa K1073022"
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'''iGEM Team Braunschweig 2013:''' | '''iGEM Team Braunschweig 2013:''' | ||
| − | '''Left:''' Liquid cultures of ''E. coli XL1'' expressing chromoproteins amilGFP (<partinfo>BBa_K1073024</partinfo>), aeBlue (<partinfo>BBa_K1073020</partinfo>) | + | '''Left:''' Liquid cultures of ''E. coli XL1'' expressing chromoproteins amilGFP (<partinfo>BBa_K1073024</partinfo>), eforRed and aeBlue (<partinfo>BBa_K1073020</partinfo>). |
'''Right:''' Cell pellet of ''E. coli XL1'' expressing eforRed. | '''Right:''' Cell pellet of ''E. coli XL1'' expressing eforRed. | ||
Revision as of 14:01, 27 September 2013
Constitutively expressed chromoprotein eforRed
The device consists of a strong promoter (BBa_J23100) combined with RBS (BBa_B0032) and the encoding region of the chromoprotein eforRed. This results in a constitutively high expression of eforRed.
Usage and Biology
During cultivation the chromoprotein eforRed is expressed and stored intracellular. The red color of the protein can be seen by naked eye, thus you can use the chromoprotein as a reporter or selection marker and seperate cells easily.
iGEM Team Braunschweig 2013:
Left: Liquid cultures of E. coli XL1 expressing chromoproteins amilGFP (BBa_K1073024), eforRed and aeBlue (BBa_K1073020).
Right: Cell pellet of E. coli XL1 expressing eforRed.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]