Difference between revisions of "Part:BBa K1189018"

Revision as of 08:04, 27 September 2013

Human ferritin di-subunit with E coil w/ LacI promoter

This part was created by fusing the heavy chain and light chains (BBa_K1189024 BBa_K1189025) of human ferritin together. It is expressed under the lacI promoter (BBa_J04500) and has a his-tag for protein purification. An E-coil (BBa_K1189011) is included in order to allow binding of parts containing the respective K-coil (BBa_K1189010).

This construct can be used as a reporter through a modification of the iron core to form Prussian Blue.

UCalgary2013TRPrussianblueferritinsynthesispartspage.png

Team:Calgary Comparison image of commercial ferritin to Prussian blue ferritin after the synthesis reaction. The synthesis reaction took place over a 12 hour time period.

The resulting molecule can then catalyze the formation of radicals from hydrogen peroxide, which can then cause a colour change in substrates such as TMB or ABTS.

Team:Calgary Image of the colours of ABTS and TMB (10 mg/mL for both) after reacting with Prussian blue ferritin.

Something about scaffold…

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 1289

@@ Line 8: / Line 8: @@
 	<p><b>Team:Calgary</b> Comparison image of commercial ferritin to Prussian blue ferritin after the synthesis reaction. The synthesis reaction took place over a 12 hour time period. </p>
 <br>
-<p> The resulting molecuel can then catalyse the formation of radicals from hydrogen perxoide, which can then cause a colour change in substrates such as TMB or ABTS.
+<p> The resulting molecule can then catalyze the formation of radicals from hydrogen peroxide, which can then cause a colour change in substrates such as TMB or ABTS.
 <br>
 https://static.igem.org/mediawiki/2013/6/6c/UCalgary2013TRSubstratecolourpartspage.png