Difference between revisions of "Part:BBa K1189019"

 
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<partinfo>BBa_K1189019 short</partinfo>
 
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<p>This heavy ferritin chain comes from humans. This part along with light ferritin (<partinfo>BBa_K1189024</partinfo>), form the ferritin nanoparticle, an iron-storage particle made up of 24 subunits. The formed nanoparticle is highly robust, remaining stable at extreme pHs and temperatures. The difference between light ferritin is that this chain contains a ferroxidase centre.
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===Usage and Biology===
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This nanoparticle can also be used as a reporter when the iron core is modified with potassium ferrocyanide to form Prussian Blue. The Prussian Blue ferritin can then act as a peroxidase mimic, similar to horseradish peroxidase, resulting in colour changes in the presence of hydrogen peroxide, and TMB or ABTS.
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We added the lacI promoter (<partinfo>BBa_J04500</partinfo>),  double terminator (<partinfo>BBa_B0010</partinfo>, & <partinfo>BBa_B0012</partinfo>) and a his-tag in order for us to induce protein expression as well as purify it.
  
 
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Revision as of 07:46, 27 September 2013

Heavy chain human ferritin with his-tag and E-coil under the lacI inducible promoter

This heavy ferritin chain comes from humans. This part along with light ferritin (BBa_K1189024), form the ferritin nanoparticle, an iron-storage particle made up of 24 subunits. The formed nanoparticle is highly robust, remaining stable at extreme pHs and temperatures. The difference between light ferritin is that this chain contains a ferroxidase centre.

This nanoparticle can also be used as a reporter when the iron core is modified with potassium ferrocyanide to form Prussian Blue. The Prussian Blue ferritin can then act as a peroxidase mimic, similar to horseradish peroxidase, resulting in colour changes in the presence of hydrogen peroxide, and TMB or ABTS.

We added the lacI promoter (BBa_J04500), double terminator (BBa_B0010, & BBa_B0012) and a his-tag in order for us to induce protein expression as well as purify it. Sequence and Features

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 230
    Illegal AgeI site found at 905
  • 1000
    COMPATIBLE WITH RFC[1000]