Difference between revisions of "Part:BBa K1073022:Experience"

Revision as of 20:28, 26 September 2013

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Applications of BBa_K1073022

When eforRed is expressed the colonies on agar plates as well as cells liquid culture exhibit a bright pink color. The iGEM Team Braunschweig 2013 used this device to differentiate two different strains when incubated in a mixed culture.

User Reviews

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BBa_K1073022 iGEM Team Braunschweig 2013

The chromoprotein eforRed has a distinct red color. In order to avoid absoption of the chomoproteins during measurement with the photometer, the absorption and emission spectra were measured.

Braunschweig 2013: Absorptionspectrum of chromoprotein eforRed in the wavelength range 400-800 nm.

Absorption maximum for eforRed: 534 nm

Emission maximum for eforRed: 605,4 nm

Accordingly the photometer measurements for growth curves were carried out by 520 nm with respect to the chromoproteins amilGFP (BBa_K1073024) and aeBlue (BBa_K1073020).

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 ===Applications of BBa_K1073022===
-When eforRed is expressed, the colonies on plates as well as the liquid culture become red. The iGEM Team Braunschweig 2013 used this device to differentiate two different strains from one approach.
+When eforRed is expressed the colonies on agar plates as well as cells liquid culture exhibit a bright pink color. The iGEM Team Braunschweig 2013 used this device to differentiate two different strains when incubated in a mixed culture.
 ===User Reviews===