Difference between revisions of "Part:BBa K1073020:Experience"
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The chromoprotein aeBlue exhibits a distinct blue color when expressed. In order to avoid absoption of the chomoproteins during measurement with the photometer the absorption and emission spectra were measured. | The chromoprotein aeBlue exhibits a distinct blue color when expressed. In order to avoid absoption of the chomoproteins during measurement with the photometer the absorption and emission spectra were measured. | ||
| − | [[File:AeBlue.jpg | | + | [[File:AeBlue.jpg |400px]] |
'''iGEM Team Braunschweig 2013:''' Absorption spectrum of chromoprotein aeBlue in a wavelength range of 400-800 nm. | '''iGEM Team Braunschweig 2013:''' Absorption spectrum of chromoprotein aeBlue in a wavelength range of 400-800 nm. | ||
| − | Absorption maximum for aeBlue: 595 nm | + | '''Absorption maximum for aeBlue:''' 595 nm |
| − | [[File:BS2013Emission aeBlue.jpg | | + | [[File:BS2013Emission aeBlue.jpg |400px]] |
'''iGEM Team Braunschweig 2013:'''Emission spectrum of chromoprotein aeBlue. This chromoprotein does not show any significant emission in a wavelength range between 500-800 nm and therefore cannot be detected by its fluorescence. | '''iGEM Team Braunschweig 2013:'''Emission spectrum of chromoprotein aeBlue. This chromoprotein does not show any significant emission in a wavelength range between 500-800 nm and therefore cannot be detected by its fluorescence. | ||
| − | + | ''' | |
| − | Emission maximum for aeBlue: none (dark quencher) | + | Emission maximum for aeBlue:''' none (dark quencher) |
AeBlue is a so called dark quencher protein. When excited this kind of chromophores relax back to the ground state without emitting energy through radiation but remain dark [Le Reste et al., 2012]. | AeBlue is a so called dark quencher protein. When excited this kind of chromophores relax back to the ground state without emitting energy through radiation but remain dark [Le Reste et al., 2012]. | ||
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Accordingly the photometer measurements for growth curves were carried out by 520 nm with respect to the chromoproteins amilGFP (<partinfo>BBa_K1073024</partinfo>) and eforRed (<partinfo>BBa_K1073022</partinfo>). | Accordingly the photometer measurements for growth curves were carried out by 520 nm with respect to the chromoproteins amilGFP (<partinfo>BBa_K1073024</partinfo>) and eforRed (<partinfo>BBa_K1073022</partinfo>). | ||
Revision as of 20:08, 26 September 2013
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Applications of BBa_K1073020
When aeBlue is expressed, the colonies on plates as well as the liquid culture become blue. The iGEM Team Braunschweig 2013 used this device to differentiate two different strains from one approach.
User Reviews
UNIQc45c3c1e1dd3fe2f-partinfo-00000000-QINU
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iGEM Team Braunschweig 2013 |
The chromoprotein aeBlue exhibits a distinct blue color when expressed. In order to avoid absoption of the chomoproteins during measurement with the photometer the absorption and emission spectra were measured.
iGEM Team Braunschweig 2013: Absorption spectrum of chromoprotein aeBlue in a wavelength range of 400-800 nm. Absorption maximum for aeBlue: 595 nm
iGEM Team Braunschweig 2013:Emission spectrum of chromoprotein aeBlue. This chromoprotein does not show any significant emission in a wavelength range between 500-800 nm and therefore cannot be detected by its fluorescence. Emission maximum for aeBlue: none (dark quencher) AeBlue is a so called dark quencher protein. When excited this kind of chromophores relax back to the ground state without emitting energy through radiation but remain dark [Le Reste et al., 2012]. Accordingly the photometer measurements for growth curves were carried out by 520 nm with respect to the chromoproteins amilGFP (BBa_K1073024) and eforRed (BBa_K1073022). |
UNIQc45c3c1e1dd3fe2f-partinfo-00000004-QINU