Difference between revisions of "Part:BBa K1073020:Experience"

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<I>iGEM Team Braunschweig 2013</I>
 
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Revision as of 19:37, 26 September 2013


This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K1073020

When aeBlue is expressed, the colonies on plates as well as the liquid culture become blue. The iGEM Team Braunschweig 2013 used this device to differentiate two different strains from one approach.

User Reviews

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iGEM Team Braunschweig 2013

The chromoprotein aeBlue has a distinct blue color. In order to avoid absoption of the chomoproteins during measurement with the photometer, the absorption and emission spectra were measured.

AeBlue.jpg

Braunschweig 2013: Absorptionspectrum of chromoprotein aeBlue in the wavelength range 400-800 nm.


Absorption maximum for aeBlue: 595 nm

Emission maximum for aeBlue: none (dark quencher)


Accordingly the photometer measurements for growth curves were carried out by 520 nm with respect to the chromoproteins amilGFP (BBa_K1073024) and eforRed (BBa_K1073022).

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