Difference between revisions of "Part:BBa C0040:Design"

 
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_C0040 short</partinfo>
 
<partinfo>BBa_C0040 short</partinfo>
  
 
<partinfo>BBa_C0040 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_C0040 SequenceAndFeatures</partinfo>
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===Design Notes===
 
===Design Notes===
  
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References (unparsed) here: <p>Elowitz, M. B. Transport, Assembly, and Dynamics in Systems of Interacting      Proteins. Thesis, Princeton Univ., Princeton (1999). </P> <p> Lutz R, Bujard H., Independent and tight regulation of transcriptional units in Escherichia coli via the LacR/O, the TetR/O and AraC/I1-I2 regulatory elements. Nucleic Acids Res. 1997 Mar 15;25(6):1203-10.  PMID: 9092630 </p> <P> References (unparsed) here: <p>Elowitz, M. B. Transport, Assembly, and Dynamics in Systems of Interacting      Proteins. Thesis, Princeton Univ., Princeton (1999). </P> <p> Lutz R, Bujard H., Independent and tight regulation of transcriptional units in Escherichia coli via the LacR/O, the TetR/O and AraC/I1-I2 regulatory elements. Nucleic Acids Res. 1997 Mar 15;25(6):1203-10.  PMID: 9092630 </p> <P>BBa_C0040 TetR Protein is based on the TetR sequence from Elowitz's repressilator.      It has been modified to include a rapid degradation LVA tail, and includes      the BioBrick standard assembly head and tail restriction sites. The RBS has      been removed. The stop codon has been changed from TAA to a double stop codon      TAATAA.  <P>
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BBa_C0040 TetR Protein is based on the TetR sequence from Elowitz's repressilator.      It has been modified to include a rapid degradation [[Help:Tag|LVA tail]], and includes      the BioBrick standard assembly head and tail restriction sites. The RBS has      been removed. The stop codon has been changed from TAA to a double stop codon      <b>TAATAA</b>. 
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===Source===
 
===Source===
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*Elowitz, M. B. Transport, Assembly, and Dynamics in Systems of Interacting Proteins. Thesis, Princeton Univ., Princeton (1999)
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*Organism: [http://www3.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?lvl=0&id=562 Escherichia Coli]
  
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--What is the source of this part?--
 
  
 
===References===
 
===References===
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*NCBI accession and version #: [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=Protein&dopt=GenPept&list_uids=g42494906  AAS17729.1  GI:42494906]
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*UniProt accession #: [http://www.pir.uniprot.org/cgi-bin/upEntry?id=Q6QR72_ECOLI Q6QR72]
  
  
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===Files===
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*Elowitz, M. B. Transport, Assembly, and Dynamics in Systems of Interacting      Proteins. Thesis, Princeton Univ., Princeton (1999). 
  
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* kill this file : -- No description --
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*Lutz R, Bujard H., Independent and tight regulation of transcriptional units in Escherichia coli via the LacR/O, the TetR/O and AraC/I1-I2 regulatory elements. Nucleic Acids Res. 1997 Mar 15;25(6):1203-10.  PMID: 9092630

Latest revision as of 23:36, 19 July 2006

tetracycline repressor from transposon Tn10 (+LVA)


Barcodes are discontinued, but one was appended to the sequence of this part. Composite parts using this part will include the barcode. More ...

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

BBa_C0040 TetR Protein is based on the TetR sequence from Elowitz's repressilator. It has been modified to include a rapid degradation LVA tail, and includes the BioBrick standard assembly head and tail restriction sites. The RBS has been removed. The stop codon has been changed from TAA to a double stop codon TAATAA.


Source

  • Elowitz, M. B. Transport, Assembly, and Dynamics in Systems of Interacting Proteins. Thesis, Princeton Univ., Princeton (1999)
  • Organism: [http://www3.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?lvl=0&id=562 Escherichia Coli]


References

  • NCBI accession and version #: [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=Protein&dopt=GenPept&list_uids=g42494906 AAS17729.1 GI:42494906]
  • UniProt accession #: [http://www.pir.uniprot.org/cgi-bin/upEntry?id=Q6QR72_ECOLI Q6QR72]


  • Elowitz, M. B. Transport, Assembly, and Dynamics in Systems of Interacting Proteins. Thesis, Princeton Univ., Princeton (1999).
  • Lutz R, Bujard H., Independent and tight regulation of transcriptional units in Escherichia coli via the LacR/O, the TetR/O and AraC/I1-I2 regulatory elements. Nucleic Acids Res. 1997 Mar 15;25(6):1203-10. PMID: 9092630