Difference between revisions of "Part:BBa K1045017:Design"

(→‎Design Notes)
(→‎Design Notes)
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===Design Notes===
 
===Design Notes===
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DarR fused to the terminator were cut out from part [[Part:BBa_K1045016|BBa_K1045016]] and ligated in a pre-fixing composition into part [[Part:BBa_K1045015|BBa_K1045015]].
 
DarR fused to the terminator were cut out from part [[Part:BBa_K1045016|BBa_K1045016]] and ligated in a pre-fixing composition into part [[Part:BBa_K1045015|BBa_K1045015]].
  

Revision as of 11:18, 25 September 2013

DarR reporter system


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 806
    Illegal NheI site found at 829
    Illegal NheI site found at 909
    Illegal NheI site found at 932
    Illegal NotI site found at 718
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 731
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 860
    Illegal AgeI site found at 168
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1630


Design Notes

DarR fused to the terminator were cut out from part BBa_K1045016 and ligated in a pre-fixing composition into part BBa_K1045015.

Source

To be continued

References