Difference between revisions of "Part:BBa K1184000:Experience"
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[[image:KillerRed_RFP_Phototoxicity.png|thumb|600px|<b>Figure 1:</b> Phototoxicity of KillerRed and mRFP1 (<partinfo>BBa_E1010</partinfo>) on <i>E. coli</i> XL10 by irradiating with a HBO100 lamp (includes 375 nm LP filter) for 5 hours.]] | [[image:KillerRed_RFP_Phototoxicity.png|thumb|600px|<b>Figure 1:</b> Phototoxicity of KillerRed and mRFP1 (<partinfo>BBa_E1010</partinfo>) on <i>E. coli</i> XL10 by irradiating with a HBO100 lamp (includes 375 nm LP filter) for 5 hours.]] | ||
− | <p>KillerRed's phototoxic effect on <i>E. coli</i> XL10 is shown in Figure 1. </p> | + | <p>KillerRed's phototoxic effect on <i>E. coli</i> XL10 is shown in Figure 1.<br /> |
+ | RFP 66% ± 20% <br /> | ||
+ | KillerRed: 51% ± 17% culture alive after photobleaching | ||
+ | </p> | ||
[[image:KR-Photobleach.png|thumb|600px|<b>Figure 1:</b> Photobleaching curve of KillerRed with a HBO100 mercury-arc lamp]] | [[image:KR-Photobleach.png|thumb|600px|<b>Figure 1:</b> Photobleaching curve of KillerRed with a HBO100 mercury-arc lamp]] | ||
<p>XL10 Ultracompetent cells were transformed with <partinfo>BBa_K1184000</partinfo> with <partinfo>BBa_R0010</partinfo> as the wild-type lac promoter and induced overnight with IPTG.The overnight was bleached for 180 minutes with HBO100 (100W Mercury-arc lamp). Fluorescence data was taken using a Tecan Safire II with the parameters shown in Table 1. Fluorescence Data is shown in Table 2.</p> | <p>XL10 Ultracompetent cells were transformed with <partinfo>BBa_K1184000</partinfo> with <partinfo>BBa_R0010</partinfo> as the wild-type lac promoter and induced overnight with IPTG.The overnight was bleached for 180 minutes with HBO100 (100W Mercury-arc lamp). Fluorescence data was taken using a Tecan Safire II with the parameters shown in Table 1. Fluorescence Data is shown in Table 2.</p> |
Revision as of 18:19, 14 September 2013
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Applications of BBa_K1184000
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Carnegie_Mellon 2013 |
KillerRed's phototoxic effect on E. coli XL10 is shown in Figure 1. XL10 Ultracompetent cells were transformed with BBa_K1184000 with BBa_R0010 as the wild-type lac promoter and induced overnight with IPTG.The overnight was bleached for 180 minutes with HBO100 (100W Mercury-arc lamp). Fluorescence data was taken using a Tecan Safire II with the parameters shown in Table 1. Fluorescence Data is shown in Table 2.
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