Difference between revisions of "Part:BBa K1184000:Experience"
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| + | <partinfo>BBa_K1184000 AddReview 3</partinfo> | ||
| + | <I>Carnegie_Mellon 2013</I> | ||
| + | |width='60%' valign='top'| | ||
| + | [[image:KillerRed_RFP_Phototoxicity.png|thumb|600px|<b>Figure 1:</b> Phototoxicity of KillerRed and mRFP1 (<partinfo>BBa_E1010</partinfo>) on <i>E. coli</i> XL10 by irradiating with a HBO100 lamp (includes 375 nm LP filter) for 5 hours.]] | ||
| + | <p>KillerRed's phototoxic effect on <i>E. coli</i> XL10 is shown in Figure 1. </p> | ||
| + | [[image:KR-Photobleach.png|thumb|600px|<b>Figure 1:</b> Photobleaching curve of KillerRed with a HBO100 mercury-arc lamp]] | ||
| + | <p>XL10 Ultracompetent cells were transformed with <partinfo>BBa_K1184000</partinfo> with <partinfo>BBa_R0010</partinfo> as the wild-type lac promoter and induced overnight with IPTG.The overnight was bleached for 180 minutes with HBO100 (100W Mercury-arc lamp). Fluorescence data was taken using a Tecan Safire II with the parameters shown in Table 1. Fluorescence Data is shown in Table 2.</p> | ||
| + | <table cellpadding="2" border="1px" cellspacing="0" align="center" width="70%"> | ||
| + | <caption><p align="justify"><b>Table 1</b> Tecan Safire<sup>2</sup> Parameters</p></caption> | ||
| + | <tr><td><b>Excitation (nm)</b></td><td>585</td></tr> | ||
| + | <tr><td><b>Emission (nm)</b></td><td>610</td></tr> | ||
| + | <tr><td><b>Excitation bandwidth (nm)</b></td><td>10</td></tr> | ||
| + | <tr><td><b>Emission bandwidth (nm)</b></td><td>10</td></tr> | ||
| + | <tr><td><b>Gain</b></td><td>129</td></tr> | ||
| + | <tr><td><b>Number of reads</b></td><td>10</td></tr> | ||
| + | <tr><td><b>Integration Time (microseconds)</b></td><td>40</td></tr> | ||
| + | </table> | ||
| + | <table cellpadding="2" border="1px" cellspacing="0" align="center" width="50%"> | ||
| + | <caption><p align="justify"><b>Table 2</b> Shows the fluorescence data over time during photobleaching.</p></caption> | ||
| + | <tr><td align="center"><b>Time (minutes)</b></td><td align="center"><b>Fluorescence (RFU)</b></td> | ||
| + | <tr><td align="left">0</td><td align="right">42598</td></tr> | ||
| + | <tr><td align="left">20</td><td align="right">37616</td></tr> | ||
| + | <tr><td align="left">40</td><td align="right">33749</td></tr> | ||
| + | <tr><td align="left">60</td><td align="right">29059</td></tr> | ||
| + | <tr><td align="left">80</td><td align="right">25680</td></tr> | ||
| + | <tr><td align="left">100</td><td align="right">21985</td></tr> | ||
| + | <tr><td align="left">120</td><td align="right">19442</td></tr> | ||
| + | <tr><td align="left">140</td><td align="right">17031</td></tr> | ||
| + | <tr><td align="left">160</td><td align="right">15738</td></tr> | ||
| + | <tr><td align="left">180</td><td align="right">13741</td></tr> | ||
| + | </table> | ||
| + | |}; | ||
Revision as of 17:02, 14 September 2013
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Carnegie_Mellon 2013 |
 Figure 1: Phototoxicity of KillerRed and mRFP1 (BBa_E1010) on E. coli XL10 by irradiating with a HBO100 lamp (includes 375 nm LP filter) for 5 hours. KillerRed's phototoxic effect on E. coli XL10 is shown in Figure 1. XL10 Ultracompetent cells were transformed with BBa_K1184000 with BBa_R0010 as the wild-type lac promoter and induced overnight with IPTG.The overnight was bleached for 180 minutes with HBO100 (100W Mercury-arc lamp). Fluorescence data was taken using a Tecan Safire II with the parameters shown in Table 1. Fluorescence Data is shown in Table 2.
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