Difference between revisions of "Part:BBa K1065105"

 
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<partinfo>BBa_K1065105 short</partinfo>
 
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SAM synthetase enzyme catalyzes the reaction of synthesis of S-AdoMet. RBS is 'AAGGAGG'.  
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SAM synthetase enzyme catalyzes the reaction of synthesis of S-adenosyl-L-methionine (S-AdoMet, a.k.a. <i>SAM</i>). This reaction is part of the ethylene biosynthesis pathway I, which is present in plants <a href="https://parts.igem.org/Part:BBa_K1065104:Design#References">[1]</A>.<br/>
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<center>ATP + L-Met + H2O -> S-AdoMet + Pi + PPi <a href="https://parts.igem.org/Part:BBa_K1065104:Design#References">[2]</a></center>
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S-AdoMet is an intermediate in the ethylene biosynthesis pathway I (plants), a methyl donor for many cellular molecules (Methylated Acceptors) and in the methyl salicylate (MeSA) biosynthesis pathway, and the precursor of the polyamine synthesis pathway (Spermidine/Spermine biosynthesis pathway) <a href="https://parts.igem.org/Part:BBa_K1065104:Design#References">[3]</a>.<br/>
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<span class='h3bb'>Sequence and Features</span>
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<span class='h3bb' style="font-weight: bold; font-size: 1.2em;">Sequence and Features</span>
 
<partinfo>BBa_K1065105 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K1065105 SequenceAndFeatures</partinfo>
  

Revision as of 08:53, 15 August 2013

lac_promoter + RBS + SAM synthetase

SAM synthetase enzyme catalyzes the reaction of synthesis of S-adenosyl-L-methionine (S-AdoMet, a.k.a. SAM). This reaction is part of the ethylene biosynthesis pathway I, which is present in plants [1].

ATP + L-Met + H2O -> S-AdoMet + Pi + PPi [2]

S-AdoMet is an intermediate in the ethylene biosynthesis pathway I (plants), a methyl donor for many cellular molecules (Methylated Acceptors) and in the methyl salicylate (MeSA) biosynthesis pathway, and the precursor of the polyamine synthesis pathway (Spermidine/Spermine biosynthesis pathway) [3].

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 324
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 904
  • 1000
    COMPATIBLE WITH RFC[1000]