Difference between revisions of "Part:BBa K902041:Design"
| Line 12: | Line 12: | ||
===Source=== | ===Source=== | ||
| − | Part J13002 from the registry was constructed in front of an AmdA coding | + | Part J13002 from the registry was constructed in front of an AmdA coding sequence that was amplified from Rhodococcus Erythropolis genomic prep via PCR. |
===References=== | ===References=== | ||
Pavlova,J. and Maksimov,A. The ability of Rhodococcus erythropolis to transform amides. Laboratory of Chemical Mutagenesis,Institute of Ecology and Genetics of Microorganisms, Ural Branch of RAS, 13 Golev Street, Perm 614000, Russia. 2011 | Pavlova,J. and Maksimov,A. The ability of Rhodococcus erythropolis to transform amides. Laboratory of Chemical Mutagenesis,Institute of Ecology and Genetics of Microorganisms, Ural Branch of RAS, 13 Golev Street, Perm 614000, Russia. 2011 | ||
Revision as of 00:09, 27 October 2012
Constitutive AmdA Generator
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 670
Illegal XhoI site found at 162 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 170
Illegal NgoMIV site found at 1375
Illegal NgoMIV site found at 1481
Illegal AgeI site found at 548
Illegal AgeI site found at 732 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 406
Illegal BsaI site found at 947
Design Notes
Please refer to the part design for the AmdA coding sequence.
Source
Part J13002 from the registry was constructed in front of an AmdA coding sequence that was amplified from Rhodococcus Erythropolis genomic prep via PCR.
References
Pavlova,J. and Maksimov,A. The ability of Rhodococcus erythropolis to transform amides. Laboratory of Chemical Mutagenesis,Institute of Ecology and Genetics of Microorganisms, Ural Branch of RAS, 13 Golev Street, Perm 614000, Russia. 2011