Difference between revisions of "Part:BBa K801062:Design"

Latest revision as of 11:27, 16 October 2012

(+)-limonene synthase 1 expression cassette for yeast

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 2127
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 211
Illegal SapI.rc site found at 561

This is a composite part consisting of the yeast TEF1 promoter (BBa_K319003), the Citrus limon (+)-limonene synthase 1 coding region (BBa_K801061) and the yeast CYC1 terminator (BBa_K122003).

Keywords:

Abbreviations:

Design Notes

Related BioBrick:

Quality control measures:

Backbone:

Backbone name: pSB1C3
Resistance: Cp
Copynumber: high

Protein coding:

BioBrick encodes (+)-limonene synthase
UniProt entry: [http://www.uniprot.org/uniprot/Q8L5K3 Q8L5K3]

Enzymatic activity:

E.C. Number: [http://enzyme.expasy.org/EC/4.2.3.20 4.2.3.20]

Cytotoxicity:
cytotoxic for S. cervisiaegrowth inhibition at 1 mM, lethal effect at 100 mM

Safety notes:

Intellectual property:

Corresponding part author/authors:

Source

Source:

BioBrick: BBa_I742111

Organism:

Gene sequence derived from Citrus limon

References

Literature references:

http://www.ncbi.nlm.nih.gov/pubmed/12084056 Lücker et al., 2002 Lücker, J., El Tamer, M. K., Schwab, W., Verstappen, F. W. A., van der Plas, L. H. W., Bouwmeester, H. J., and Verhoeven, H. A. (2002). Monoterpene biosynthesis in lemon (Citrus limon). cDNA isolation and functional analysis of four monoterpene synthases. Eur J Biochem, 269(13):3160–71.

Database references:

@@ Line 40: / Line 40: @@
 '''Backbone:'''<br>
-<!--*Backbone name: pSB1C3'/?backbone_name?-->
+* Backbone name: pSB1C3
-<!--*Resistance: Amp/Cp/Kan/Tet-->
+* Resistance: Cp
-<!--*Copynumber: low/medium/high-->
+* Copynumber: high
 '''Protein coding:'''<br>
-<!--*Protein: ?Name_of_gene_product? [Nucleotide 1 to ???]-->
+* BioBrick encodes (+)-limonene synthase
-<!--*The protein has the amino acid replacements ???99??? to ???99???.-->
+* UniProt entry: [http://www.uniprot.org/uniprot/Q8L5K3 Q8L5K3]
-<!--*The protein encoded is posttranslationally modified by ???.-->
+<!--none/not known/cytotoxic for ''organism name''-->
-<!--*Tag: n-terminally fused/c-terminally fused His5/His6/Strep/Flag/other-->
 '''Enzymatic activity:'''
-<!--none/EC-number ?.?.?.?-->
+* E.C. Number: [http://enzyme.expasy.org/EC/4.2.3.20 4.2.3.20]
 '''Cytotoxicity:'''<br>
-<!--none/not known/cytotoxic for ''organism name''-->
+cytotoxic for ''S. cervisiae''growth inhibition at 1 mM, lethal effect at 100 mM
 '''Safety notes:'''<br>
@@ Line 70: / Line 69: @@
 '''Source:'''<br>
+*BioBrick: BBa_I742111
 <!--*Commercial system: plasmid name, system name, company name-->
 <!--*Plasmid: p???, provided by ?name_of_person?, ?institute/university?, ?country?-->
@@ Line 80: / Line 80: @@
 '''Organism:'''<br>
+* Gene sequence derived from ''Citrus limon''
 <!--*Genesequence derived from ''?organism_name?''-->
 <!--*Codonoptimized for ''?organism_name?''-->
 <!--*Designed for the following Chassis: ''?organism-name?''-->
 <!--*Statement about functionality in other chassis.-->
 ===References===
@@ Line 90: / Line 90: @@
 '''Literature references:'''<br>
+*[[http://www.ncbi.nlm.nih.gov/pubmed/12084056 Lücker et al., 2002]] Lücker, J., El Tamer, M. K., Schwab, W., Verstappen, F. W. A., van der Plas, L. H. W., Bouwmeester, H. J., and Verhoeven, H. A. (2002). Monoterpene biosynthesis in lemon (''Citrus limon''). cDNA isolation and functional analysis of four monoterpene synthases. ''Eur J Biochem'', 269(13):3160–71.
 <!--*[http://www.ncbi.nlm.nih.gov/pubmed/?PMID? '''Pubmed:''' ?Author(s)?, ?year?: ?title?]-->