Difference between revisions of "Part:BBa K801062:Design"

 
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K801062 short</partinfo>
 
<partinfo>BBa_K801062 short</partinfo>
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<partinfo>BBa_K801062 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K801062 SequenceAndFeatures</partinfo>
  
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This is a composite part consisting of the yeast TEF1 promoter (BBa_K319003), the ''Citrus limon'' (+)-limonene synthase 1 coding region (BBa_K801061) and the yeast CYC1 terminator (BBa_K122003).
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<br>'''Keywords:'''
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<!--These keywords are necessary to find your part using a fulltext sarch.-->
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<!--keyword_1, keyword_2, keyword_3, keyword_4, keyword_5-->
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<br>'''Abbreviations:'''
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<!--*used_abbreviation_1 = full_name_of_used_abbreviations_1-->
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<!--*used_abbreviation_2 = full_name_of_used_abbreviations_2-->
  
 
===Design Notes===
 
===Design Notes===
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-
 
  
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'''Related BioBrick:'''
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* [https://parts.igem.org/Part:BBa_K801060 BBa_K801060: (+)-Limonene synthase 1 with Strep-tag and yeast consensus sequence]
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* [https://parts.igem.org/Part:BBa_K801061 BBa_K801061: (+)-Limonene synthase 1 coding region]
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* [https://parts.igem.org/Part:BBa_K801063 BBa_K801063: (+)-limonene synthase 1 expression cassette for yeast]
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* [https://parts.igem.org/Part:BBa_K801064 BBa_K801064: (+)-limonene synthase 1 expression cassette for yeast]
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* [https://parts.igem.org/Part:BBa_K801065 BBa_K801065: (+)-limonene synthase 1 with Strep-Tag]
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* [https://parts.igem.org/Part:BBa_K801066 BBa_K801066: (+)-limonene synthase 1 with Yeast Consensus Sequence for improved expression]
  
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<!--*Other versions:[https://parts.igem.org/wiki/index.php?title=Part:BBa_?????? BBa_??????: Name_of_part] -->
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<!--*Related BioBricks:[https://parts.igem.org/wiki/index.php?title=Part:BBa_?????? BBa_??????: Name_of_part]
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'''Cloning details:'''<br>
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<!--*Designed in RFC10/RFC23/RFC25/RFC25_N-part-->
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<!--*Mutation C889G to delete XbaI restriction site-->
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<!--*Truncation upstream/downstream compared to template, ?explanation?-->
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'''Quality control measures:'''<br>
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<!--*Test digestion using ?enzyme1? & ?enzyme2?/Not yet performed-->
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<!--*Sequencing using primer ?primer_name?/Not yet sequenced-->
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<!--*Part was partly sequenced/Part was totally sequenced-->
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'''Backbone:'''<br>
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* Backbone name: pSB1C3
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* Resistance: Cp
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* Copynumber: high
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'''Protein coding:'''<br>
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* BioBrick encodes (+)-limonene synthase
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* UniProt entry: [http://www.uniprot.org/uniprot/Q8L5K3 Q8L5K3]
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<!--none/not known/cytotoxic for ''organism name''-->
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'''Enzymatic activity:'''
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* E.C. Number: [http://enzyme.expasy.org/EC/4.2.3.20 4.2.3.20]
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'''Cytotoxicity:'''<br>
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cytotoxic for ''S. cervisiae''growth inhibition at 1 mM, lethal effect at 100 mM
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'''Safety notes:'''<br>
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<!--Known and anticipated sefety issues: none/health_risk/environmental_risk/other_risk-->
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<!--Known and anticipated security issues: none/other.-->
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'''Intellectual property:'''
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<!--Information on patent situation.-->
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<!--Intellectual property claims made by the authors.-->
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'''Corresponding part author/authors:'''
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<!--https://igem.org/User_Information.cgi?user_id=????/email-->
  
 
===Source===
 
===Source===
  
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This is a composite part consisting of the yeast TEF1 promoter (BBa_K319003), the ''Citrus limon'' (+)-limonene synthase 1 coding region (BBa_K801061) and the yeast CYC1 terminator (BBa_K122003).
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'''Source:'''<br>
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*BioBrick: BBa_I742111
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<!--*Commercial system: plasmid name, system name, company name-->
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<!--*Plasmid: p???, provided by ?name_of_person?, ?institute/university?, ?country?-->
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<!--*Preexisting BioBrick ?Bba_number?-->
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<!--*cDNA Clone: ?clone_name?, ?company_name?-->
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<!--*Synthesized by ?company_name?.-->
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<!--'''Forward Primer:'''<br><code>5'- ??? - 3'</code><br>-->
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<!--'''Reverse Primer:'''<br><code>5'- ??? - 3'</code><br>-->
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'''Organism:'''<br>
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* Gene sequence derived from ''Citrus limon''
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<!--*Genesequence derived from ''?organism_name?''-->
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<!--*Codonoptimized for ''?organism_name?''-->
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<!--*Designed for the following Chassis: ''?organism-name?''-->
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<!--*Statement about functionality in other chassis.-->
  
 
===References===
 
===References===
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<!-- Here you find templates to insert references to literature and different databases-->
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'''Literature references:'''<br>
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*[[http://www.ncbi.nlm.nih.gov/pubmed/12084056 Lücker et al., 2002]] Lücker, J., El Tamer, M. K., Schwab, W., Verstappen, F. W. A., van der Plas, L. H. W., Bouwmeester, H. J., and Verhoeven, H. A. (2002). Monoterpene biosynthesis in lemon (''Citrus limon''). cDNA isolation and functional analysis of four monoterpene synthases. ''Eur J Biochem'', 269(13):3160–71.
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<!--*[http://www.ncbi.nlm.nih.gov/pubmed/?PMID? '''Pubmed:''' ?Author(s)?, ?year?: ?title?]-->
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'''Database references:'''<br>
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<!--*[http://www.ncbi.nlm.nih.gov/nuccore/?accessNr? '''GenBank''': ?title?]-->
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<!--*[http://www.ebi.ac.uk/interpro/IEntry?ac=?accessNr? '''Interpro''': ?title?]-->
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<!--*[http://www.uniprot.org/uniprot/?accessNr? '''Uniprot''': ?title?]-->
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<!--*[http://pfam.sanger.ac.uk/family/?accessNr? '''Pfam:''' ?title?]-->
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<!--*[http://www.rcsb.org/pdb/explore/explore.do?structureId=?accessNR? '''PDB:''' ?tile?]-->
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<!--*[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=?accessNr? '''Branda:''' ?title?]-->

Latest revision as of 11:27, 16 October 2012

(+)-limonene synthase 1 expression cassette for yeast


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 2127
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 211
    Illegal SapI.rc site found at 561


This is a composite part consisting of the yeast TEF1 promoter (BBa_K319003), the Citrus limon (+)-limonene synthase 1 coding region (BBa_K801061) and the yeast CYC1 terminator (BBa_K122003).


Keywords:


Abbreviations:

Design Notes

Related BioBrick:


Quality control measures:

Backbone:

  • Backbone name: pSB1C3
  • Resistance: Cp
  • Copynumber: high

Protein coding:

  • BioBrick encodes (+)-limonene synthase
  • UniProt entry: [http://www.uniprot.org/uniprot/Q8L5K3 Q8L5K3]

Enzymatic activity:

  • E.C. Number: [http://enzyme.expasy.org/EC/4.2.3.20 4.2.3.20]

Cytotoxicity:
cytotoxic for S. cervisiaegrowth inhibition at 1 mM, lethal effect at 100 mM

Safety notes:

Intellectual property:

Corresponding part author/authors:

Source

Source:

  • BioBrick: BBa_I742111


Organism:

  • Gene sequence derived from Citrus limon

References

Literature references:

  • http://www.ncbi.nlm.nih.gov/pubmed/12084056 Lücker et al., 2002 Lücker, J., El Tamer, M. K., Schwab, W., Verstappen, F. W. A., van der Plas, L. H. W., Bouwmeester, H. J., and Verhoeven, H. A. (2002). Monoterpene biosynthesis in lemon (Citrus limon). cDNA isolation and functional analysis of four monoterpene synthases. Eur J Biochem, 269(13):3160–71.

Database references: