Difference between revisions of "Part:BBa K801061:Design"
(→Design Notes) |
Nadine1990 (Talk | contribs) (→Design Notes) |
||
| (2 intermediate revisions by the same user not shown) | |||
| Line 51: | Line 51: | ||
'''Cytotoxicity:'''<br> | '''Cytotoxicity:'''<br> | ||
| − | + | cytotoxic for ''S.cervisiae'': growth inhibition at 1 mM, lethal effect at 100 mM | |
'''Safety notes:'''<br> | '''Safety notes:'''<br> | ||
| Line 88: | Line 88: | ||
'''Literature references:'''<br> | '''Literature references:'''<br> | ||
| − | * | + | |
| − | + | *[http://www.ncbi.nlm.nih.gov/pubmed/12084056 '''Pubmed:''' Lücker, J., El Tamer, M. K., Schwab, W., Verstappen, F. W. A., van der Plas, L. H. W., Bouwmeester, H. J., and Verhoeven, H. A., 2002: Monoterpene biosynthesis in lemon (Citrus limon). cDNA isolation and functional analysis of four monoterpene synthases. Eur J Biochem, 269(13):3160–71] | |
'''Database references:'''<br> | '''Database references:'''<br> | ||
| − | + | *[http://www.ncbi.nlm.nih.gov/nucleotide/21435702 '''GenBank''': Citrus limon (+)-limonene synthase 1 mRNA, complete cds] | |
<!--*[http://www.ebi.ac.uk/interpro/IEntry?ac=?accessNr? '''Interpro''': ?title?]--> | <!--*[http://www.ebi.ac.uk/interpro/IEntry?ac=?accessNr? '''Interpro''': ?title?]--> | ||
| − | + | *[http://www.uniprot.org/uniprot/Q8L5K3 '''Uniprot''': (R)-limonene synthase 1] | |
<!--*[http://pfam.sanger.ac.uk/family/?accessNr? '''Pfam:''' ?title?]--> | <!--*[http://pfam.sanger.ac.uk/family/?accessNr? '''Pfam:''' ?title?]--> | ||
<!--*[http://www.rcsb.org/pdb/explore/explore.do?structureId=?accessNR? '''PDB:''' ?tile?]--> | <!--*[http://www.rcsb.org/pdb/explore/explore.do?structureId=?accessNR? '''PDB:''' ?tile?]--> | ||
<!--*[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=?accessNr? '''Branda:''' ?title?]--> | <!--*[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=?accessNr? '''Branda:''' ?title?]--> | ||
Latest revision as of 11:26, 16 October 2012
(+)-Limonene synthase 1 coding region
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 100
http://www.ncbi.nlm.nih.gov/nuccore/21435702
Keywords:
limonen,
Abbreviations:
Design Notes
Related BioBrick:
- BBa_K801060: (+)-Limonene synthase 1 with Strep-tag and yeast consensus sequence
- BBa_K801062: (+)-limonene synthase 1 expression cassette for yeast
- BBa_K801063: (+)-limonene synthase 1 expression cassette for yeast
- BBa_K801064: (+)-limonene synthase 1 expression cassette for yeast
- BBa_K801065: (+)-limonene synthase 1 with Strep-Tag
- BBa_K801066: (+)-limonene synthase 1 with Yeast Consensus Sequence for improved expression
Quality control measures:
Backbone:
- Backbone name: pSB1C3
- Resistance: Cp
- Copynumber: high
Protein coding:
- BioBrick encodes (+)-limonene synthase
- UniProt entry: [http://www.uniprot.org/uniprot/Q8L5K3 Q8L5K3]
Enzymatic activity:
- E.C. Number: [http://enzyme.expasy.org/EC/4.2.3.20 4.2.3.20]
Cytotoxicity:
cytotoxic for S.cervisiae: growth inhibition at 1 mM, lethal effect at 100 mM
Safety notes:
Intellectual property:
Corresponding part author/authors:
Source
Source:
- BioBrick: BBa_I742111
Organism:
- Gene sequence derived from Citrus limon
References
Literature references:
- [http://www.ncbi.nlm.nih.gov/pubmed/12084056 Pubmed: Lücker, J., El Tamer, M. K., Schwab, W., Verstappen, F. W. A., van der Plas, L. H. W., Bouwmeester, H. J., and Verhoeven, H. A., 2002: Monoterpene biosynthesis in lemon (Citrus limon). cDNA isolation and functional analysis of four monoterpene synthases. Eur J Biochem, 269(13):3160–71]
Database references:
- [http://www.ncbi.nlm.nih.gov/nucleotide/21435702 GenBank: Citrus limon (+)-limonene synthase 1 mRNA, complete cds]
- [http://www.uniprot.org/uniprot/Q8L5K3 Uniprot: (R)-limonene synthase 1]